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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 1 — Feb. 4, 2013

Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging

Joshua L. Santarpia, Yong-Le Pan, Steven C. Hill, Neal Baker, Brian Cottrell, Laura McKee, Shanna Ratnesar-Shumate, and Ronald G. Pinnick  »View Author Affiliations


Optics Express, Vol. 20, Issue 28, pp. 29867-29881 (2012)
http://dx.doi.org/10.1364/OE.20.029867


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Abstract

A laboratory system for exposing aerosol particles to ozone and rapidly measuring the subsequent changes in their single-particle fluorescence is reported. The system consists of a rotating drum chamber and a single-particle fluorescence spectrometer (SPFS) utilizing excitation at 263 nm. Measurements made with this system show preliminary results on the ultra-violet laser-induced-fluorescence (UV-LIF) spectra of single aerosolized particles of Yersinia rohdei, and of MS2 (bacteriophage) exposed to ozone. When bioparticles are exposed in the chamber the fluorescence emission peak around 330 nm: i) decreases in intensity relative to that of the 400-550 nm band; and ii) shifts slightly toward shorter-wavelengths (consistent with further drying of the particles). In these experiments, changes were observed at exposures below the US Environmental Protection Agency (EPA) limits for ozone

© 2012 OSA

OCIS Codes
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(010.1110) Atmospheric and oceanic optics : Aerosols
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Atmospheric and Oceanic Optics

History
Original Manuscript: October 25, 2012
Revised Manuscript: December 10, 2012
Manuscript Accepted: December 11, 2012
Published: December 21, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Joshua L. Santarpia, Yong-Le Pan, Steven C. Hill, Neal Baker, Brian Cottrell, Laura McKee, Shanna Ratnesar-Shumate, and Ronald G. Pinnick, "Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging," Opt. Express 20, 29867-29881 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-28-29867


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