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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 3 — Feb. 29, 2012

Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy

Lin Wang, Cvetelin Vasilev, Daniel P. Canniffe, Luke R. Wilson, C. Neil Hunter, and Ashley J. Cadby  »View Author Affiliations

Optics Express, Vol. 20, Issue 3, pp. 3311-3324 (2012)

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We report the use of a high-refractive-index aplanatic solid immersion lens (ASIL) in total internal reflection fluorescence (TIRF) microscopy. This new solid immersion total internal reflection fluorescence (SITIRF) microscopy allows highly confined surface imaging with a significantly reduced imaging depth compared with conventional TIRF microscopy. We explore the application of a high refractive index, low optical dispersion material zirconium dioxide in the SITIRF microscope and also introduce a novel system design which enables the SITIRF microscope to work either in the epi-fluorescence or TIRF modes with variable illumination angles. We use both synthetic and biological samples to demonstrate that the imaging depth in the SITIRF microscope can be confined to a few tens of nanometers. SITIRF microscopy has the advantages of performing highly selective imaging and high-resolution high-contrast imaging. Potential applications in biological imaging and future developments of SITIRF microscopy are proposed.

© 2012 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(240.0240) Optics at surfaces : Optics at surfaces
(240.6690) Optics at surfaces : Surface waves
(260.6970) Physical optics : Total internal reflection

ToC Category:

Original Manuscript: November 23, 2011
Revised Manuscript: January 5, 2012
Manuscript Accepted: January 8, 2012
Published: January 27, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Lin Wang, Cvetelin Vasilev, Daniel P. Canniffe, Luke R. Wilson, C. Neil Hunter, and Ashley J. Cadby, "Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy," Opt. Express 20, 3311-3324 (2012)

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