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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 4 — Mar. 29, 2012

Scattered light fluorescence microscopy in three dimensions

Giulia Ghielmetti and Christof M. Aegerter  »View Author Affiliations


Optics Express, Vol. 20, Issue 4, pp. 3744-3752 (2012)
http://dx.doi.org/10.1364/OE.20.003744


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Abstract

Recently, we have proposed a method to image fluorescent structures behind turbid layers at diffraction limited resolution using wave-front shaping and the memory effect. However, this was limited to a raster scanning of the wave-front shaped focus to a two dimensional plane. In applications, it can however be of great importance to be able to scan a three dimensional volume. Here we show that this can be implemented in the same setup. This is achieved by the addition of a parabolic phase shift to the shaped wave-front. Via the memory effect, this phase shift leads to a shift of the interference based focus in the z-direction, thus opening the possibility of three dimensional imaging using scattered light fluorescence microscopy. Here, we show an example of such a three dimensional image of fluorescent nano-beads taken behind a turbid layer more than 10 mean free paths thick. Finally, we discuss the differences of the scanning in the z-direction with that in the x–y plane and the corresponding possibilities and limitations of the technique.

© 2012 OSA

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(110.0180) Imaging systems : Microscopy
(110.7050) Imaging systems : Turbid media
(290.4210) Scattering : Multiple scattering

ToC Category:
Microscopy

History
Original Manuscript: December 7, 2011
Revised Manuscript: January 23, 2012
Manuscript Accepted: January 26, 2012
Published: January 31, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Giulia Ghielmetti and Christof M. Aegerter, "Scattered light fluorescence microscopy in three dimensions," Opt. Express 20, 3744-3752 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-4-3744


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References

  1. J. B. Pawley ed., Handbook of Biological Confocal Microscopy, 3rd ed. (Springer, Berlin2006). [CrossRef]
  2. F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods2, 932–940 (2005). [CrossRef] [PubMed]
  3. A. Diaspro, ed., Confocal and Two-Photon Microscopy: Foundations, Applications and Advances (Wiley-Liss, New York, 2002).
  4. C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods5, 45–47 (2008). [CrossRef]
  5. M. Wolf, M. Ferrari, and V. Quaresima, “Progress of near-infrared spectroscopy and topography for brain and muscle clinical applications,” J. Biomed. Opt.12, 062104 (2007). [CrossRef]
  6. I. M. Vellekoop and A. P. Mosk, “Focusing coherent light through opaque strongly scattering media,” Opt. Lett.32, 2309–2311 (2007). [CrossRef] [PubMed]
  7. I. M. Vellekoop and C. M. Aegerter, “Scattered light fluorescence microscopy: imaging through turbid media,” Opt. Lett.35, 1245–1247 (2010). [CrossRef] [PubMed]
  8. C. L. Hsieh, Y. Pu, R. Grange, G. Laporte, and D. Psaltis, “Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle,” Opt. Express18, 20723–20731 (2010). [CrossRef] [PubMed]
  9. M. Cui, E. J. McDowell, and C. Yang, “An in vivo study of turbidity suppression by optical phase conjugation (TSOPC) on rabbit ear,” Opt. Express18, 25–30 (2010). [CrossRef] [PubMed]
  10. M. Cui and C. Yang, “Implementation of a digital optical phase conjugation system and its application to study the robustness of turbidity suppression by phase conjugation,” Opt. Express18, 3444–3455 (2010). [CrossRef] [PubMed]
  11. G. Lerosey, J. de Rosny, A. Tourin, and M. Fink, “Focusing beyond the diffraction limit with far-field time reversal,” Science315, 1120–1122 (2007). [CrossRef] [PubMed]
  12. Z. Yaqoob, D. Psaltis, M. S. Feld, and C. Yang, “Optical phase conjugation for turbidity suppression in biological samples,” Nat. Photonics2, 110–115 (2008). [CrossRef] [PubMed]
  13. M. Fink, “Time reversed acoustics,” Phys. Today50, 34–40 (1997). [CrossRef]
  14. J. L. Thomas, F. Wu, and M. Fink, “Time reversal focusing applied to lithotripsy,” Ultras. Imag.18, 106–121 (1996). [CrossRef]
  15. S. M. Popoff, G. Lerosey, R. Carminati, M. Fink, A. Boccara, and S. Gigan, “Measuring the transmission matrix in optics: an approach to the study and control of light propagation in disordered media,” Phys. Rev. Lett.104, 100601 (2010). [CrossRef] [PubMed]
  16. S. M. Popoff, G. Lerosey, M. Fink, A. C. Boccara, and S. Gigan, “Image transmission through an opaque material,” Nat. Commun.1, 81 (2010). [CrossRef] [PubMed]
  17. I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, “Exploiting disorder for perfect focusing,” Nat. Photonics4, 320–322 (2010). [CrossRef]
  18. Y. G. Ma, S. Sahebdivan, C. K. Ong, T. Tyc, and U. Leonhardt, “Evidence for subwavelength imaging with positive refraction,” New J. Phys.13, 033016 (2011). [CrossRef]
  19. I. M. Vellekoop and A. P. Mosk, “Universal optimal transmission of light through disordered materials,” Phys. Rev. Lett.101, 120601 (2008). [CrossRef] [PubMed]
  20. D. L. Fried, “Anisoplanatism in adaptive optics,” J. Opt. Soc. Am.72, 52–61 (1982). [CrossRef]
  21. S. Feng, C. Kane, P. A. Lee, and A. D. Stone, “Correlations and fluctuations of coherent wave transmission through disordered media,” Phys. Rev. Lett.61, 834–837 (1988). [CrossRef] [PubMed]
  22. I. Freund, M. Rosenbluh, and S. Feng, “Memory effects in propagation of optical waves through disordered media,” Phys. Rev. Lett.61, 2328–2331 (1988). [CrossRef] [PubMed]
  23. I. M. Vellekoop, E. G. van Putten, A. Lagendijk, and A. P. Mosk, “Demixing light paths inside disordered metamaterials,” Opt. Express16, 67–80 (2008). [CrossRef] [PubMed]
  24. D. Akbulut, T. J. Huisman, E. G. van Putten, W. L. Vos, and A. P. Mosk, “Focusing light through random photonic media by binary amplitude modulation,” Opt. Express19, 4017–4029 (2011). [CrossRef] [PubMed]
  25. D. B. Conkey, A. M. Caravaca-Aguirre, and R. Piestun, “High-speed scattering medium characterization with application to focusing light through turbid media,” Opt. Express20, 1733–1740 (2012). [CrossRef] [PubMed]
  26. M. Cui, “A high speed wavefront determination method based on spatial frequency modulations for focusing light through random scattering media,” Opt. Express19, 2989–2995 (2011). [CrossRef] [PubMed]
  27. I. M. Vellekoop and C. M. Aegerter, “Focusing light through living tissue,” Proc. SPIE7554, 755430 (2010). [CrossRef]

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