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Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC)Mary Williard Elting, Sabrina R. Leslie, L. Stirling Churchman, Jonas Korlach, Christopher M. J. McFaul, Jason S. Leith, Michael J. Levene, Adam E. Cohen, and James A. Spudich »View Author Affiliations
Mary Williard Elting,1,2,3
Sabrina R. Leslie,4,5
L. Stirling Churchman,2,6
Jonas Korlach,7
Christopher M. J. McFaul,4
Jason S. Leith,4
Michael J. Levene,8
Adam E. Cohen,5
and James A. Spudich2,*
1Department of Applied Physics, Stanford University, Stanford, California 94305, USA 2Department of Biochemistry, Stanford University, Stanford, California 94305, USA 3Current Address: Department of Cell and Tissue Biology, University of California, San Francisco, California 94143, USA 4Department of Physics, McGill University, Montreal, QC H3A 2TA, Canada 5Departments of Chemistry and Chemical Biology and of Physics, Harvard University, Cambridge, Massachusetts 02138, USA 6Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA 7Pacific Biosciences, Menlo Park, California 94025, USA 8Department of Biomedical Engineering, Yale University, New Haven, Connecticut 06520, USA *Corresponding author: jspudich@stanford.edu |
Optics Express, Vol. 21, Issue 1, pp. 1189-1202 (2013)
http://dx.doi.org/10.1364/OE.21.001189
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Abstract
Resolving single fluorescent molecules in the presence of high fluorophore concentrations remains a challenge in single-molecule biophysics that limits our understanding of weak molecular interactions. Total internal reflection fluorescence (TIRF) imaging, the workhorse of single-molecule fluorescence microscopy, enables experiments at concentrations up to about 100 nM, but many biological interactions have considerably weaker affinities, and thus require at least one species to be at micromolar or higher concentration. Current alternatives to TIRF often require three-dimensional confinement, and thus can be problematic for extended substrates, such as cytoskeletal filaments. To address this challenge, we have demonstrated and applied two new single-molecule fluorescence microscopy techniques, linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC), for imaging the processive motion of molecular motors myosin V and VI along actin filaments. Both technologies will allow imaging in the presence of higher fluorophore concentrations than TIRF microscopy. They will enable new biophysical measurements of a wide range of processive molecular motors that move along filamentous tracks, such as other myosins, dynein, and kinesin. A particularly salient application of these technologies will be to examine chemomechanical coupling by directly imaging fluorescent nucleotide molecules interacting with processive motors as they traverse their actin or microtubule tracks.
© 2013 OSA
OCIS Codes
(000.1430) General : Biology and medicine
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: November 5, 2012
Revised Manuscript: December 14, 2012
Manuscript Accepted: December 15, 2012
Published: January 10, 2013
Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Mary Williard Elting, Sabrina R. Leslie, L. Stirling Churchman, Jonas Korlach, Christopher M. J. McFaul, Jason S. Leith, Michael J. Levene, Adam E. Cohen, and James A. Spudich, "Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC)," Opt. Express 21, 1189-1202 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-1-1189
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References
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- E. Boukobza, A. Sonnenfeld, and G. Haran, “Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy,” J. Phys. Chem. B105(48), 12165–12170 (2001). [CrossRef]
- D. T. Chiu, R. M. Lorenz, and G. D. M. Jeffries, “Droplets for ultrasmall-volume analysis,” Anal. Chem.81(13), 5111–5118 (2009). [CrossRef] [PubMed]
- J. J. Benítez, A. M. Keller, and P. Chen, “Nanovesicle trapping for studying weak protein interactions by single-molecule FRET,” Methods Enzymol.472, 41–60 (2010). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- E. M. De La Cruz, E. M. Ostap, and H. L. Sweeney, “Kinetic mechanism and regulation of myosin VI,” J. Biol. Chem.276(34), 32373–32381 (2001). [CrossRef] [PubMed]
- S. M. Block, “Kinesin motor mechanics: binding, stepping, tracking, gating, and limping,” Biophys. J.92(9), 2986–2995 (2007). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- S. R. Leslie, A. P. Fields, and A. E. Cohen, “Convex lens-induced confinement for imaging single molecules,” Anal. Chem.82(14), 6224–6229 (2010). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- J. D. Pardee and J. A. Spudich, “Purification of muscle actin,” Methods Cell Biol.24, 271–289 (1982). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- J. Wenger, P.-F. Lenne, E. Popov, H. Rigneault, J. Dintinger, and T. Ebbesen, “Single molecule fluorescence in rectangular nano-apertures,” Opt. Express13(18), 7035–7044 (2005). [CrossRef] [PubMed]
- J. A. Theriot and T. J. Mitchison, “The nucleation-release model of actin filament dynamics in cell motility,” Trends Cell Biol.2(8), 219–222 (1992). [CrossRef] [PubMed]
- R. Littlefield and V. M. Fowler, “A minor actin catastrophe,” Nat. Cell Biol.4(9), E209–E211 (2002). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- R. Carballido-López, “The bacterial actin-like cytoskeleton,” Microbiol. Mol. Biol. Rev.70(4), 888–909 (2006). [CrossRef] [PubMed]
Adachi, K.
Aitken, C. E.
Anson, M.
Ariga, T.
Arslan, S.
Baybayan, P.
Benítez, J. J.
Bettman, B.
Bibillo, A.
Bjornson, K.
Block, S. M.
Boukobza, E.
Bryant, Z.
Carballido-López, R.
Chang, A. Y.
Chaudhuri, B.
Chen, P.
Cheney, R. E.
Chiu, D. T.
Christians, F.
Chuan, P.
Churchman, L. S.
Cicero, R.
Cicero, R. L.
Clark, S.
Cohen, A. E.
Combs, P. A.
Corrie, J. E.
Craighead, H. G.
Dalal, R.
Davis, C. T.
Dawson, J. F.
De La Cruz, E. M.
Delp, S. L.
Derr, N. D.
Dewinter, A.
DeWitt, M. A.
Dintinger, J.
Dixon, J.
Dunn, A. R.
Ebbesen, T.
Eccleston, J. F.
Eid, J.
Elting, M. W.
Fehr, A.
Fengler, S. M.
Ferenczi, M. A.
Fields, A. P.
Flusberg, B. A.
Foquet, M.
Forgacs, E.
Fowler, V. M.
Fujiwara, I.
Funatsu, T.
Furuike, S.
Gaertner, A.
Goodman, B. S.
Gray, J.
Gray, J. J.
Ha, T.
Habuchi, S.
Harada, Y.
Haran, G.
Hardenbol, P.
Heiner, C.
Hester, K.
Higuchi, H.
Holden, D.
Ishii, Y.
Ishijima, A.
Ishiwata, S.
Itoh, H.
Iwane, A. H.
Jeffries, G. D. M.
Kearns, G.
Keller, A. M.
Kikumoto, M.
Kinosita, K.
Kitamura, K.
Kojima, H.
Komori, T.
Kong, X.
Korlach, J.
Kuse, R.
Lacroix, Y.
Lenne, P.-F.
Leslie, S. R.
Levene, M. J.
Liao, J. C.
Liao, J.-C.
Lin, S.
Littlefield, R.
Lorenz, R. M.
Loveland, A. B.
Lundquist, P.
Luong, K.
Lyle, J.
Ma, C.
Marks, P.
Marks, P. J.
Maxham, M.
Mehta, A. D.
Mikhailenko, S. V.
Mitchison, T. J.
Mooseker, M. S.
Murphy, D.
Murphy, D. L.
Myong, S.
Nakayama, H.
Nishikawa, S.
Nishizaka, T.
Noji, H.
Oguchi, Y.
Ohki, T.
Oiwa, K.
Okten, Z.
Okumus, B.
Olivares, A. O.
Ostap, E. M.
Otto, G.
Otto, G. A.
Pardee, J. D.
Park, H.
Park, I.
Peluso, P.
Pham, T.
Pham, T. T.
Phillips, M.
Popov, E.
Puglisi, J. D.
Qiu, W.
Rank, D.
Reck-Peterson, S. L.
Reid, G. P.
Rief, M.
Rigneault, H.
Rock, R. S.
Roitman, D. B.
Rosenfeld, S. S.
Roy, J.
Saito, K.
Sakamoto, T.
Sebra, R.
Sellers, J. R.
Selvin, P. R.
Shen, G.
Shih, W.
Sonnenfeld, A.
Sorenson, J.
Spudich, J. A.
Sweeney, H. L.
Tadakuma, H.
Takahashi, S.
Tanaka, H.
Theriot, J. A.
Tokunaga, M.
Tomaney, A.
Travers, K.
Trentham, D. R.
Trulson, M.
Turner, S.
Turner, S. W.
Uemura, S.
Vale, R. D.
van Oijen, A. M.
Vieceli, J.
Villa, E.
Walter, J. C.
Webb, M. R.
Webb, W. W.
Wegener, J.
Wenger, J.
White, H. D.
Wu, D.
Yamada, A.
Yanagida, T.
Yang, A.
Yang, Z.
Yildiz, A.
Yoshida, M.
Zaccarin, D.
Zhao, P.
Zhong, F.
Zong, A. B.
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. J. Benítez, A. M. Keller, and P. Chen, “Nanovesicle trapping for studying weak protein interactions by single-molecule FRET,” Methods Enzymol.472, 41–60 (2010). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. M. Block, “Kinesin motor mechanics: binding, stepping, tracking, gating, and limping,” Biophys. J.92(9), 2986–2995 (2007). [CrossRef] [PubMed]
- E. Boukobza, A. Sonnenfeld, and G. Haran, “Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy,” J. Phys. Chem. B105(48), 12165–12170 (2001). [CrossRef]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- R. Carballido-López, “The bacterial actin-like cytoskeleton,” Microbiol. Mol. Biol. Rev.70(4), 888–909 (2006). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. J. Benítez, A. M. Keller, and P. Chen, “Nanovesicle trapping for studying weak protein interactions by single-molecule FRET,” Methods Enzymol.472, 41–60 (2010). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- D. T. Chiu, R. M. Lorenz, and G. D. M. Jeffries, “Droplets for ultrasmall-volume analysis,” Anal. Chem.81(13), 5111–5118 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. R. Leslie, A. P. Fields, and A. E. Cohen, “Convex lens-induced confinement for imaging single molecules,” Anal. Chem.82(14), 6224–6229 (2010). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- E. M. De La Cruz, E. M. Ostap, and H. L. Sweeney, “Kinetic mechanism and regulation of myosin VI,” J. Biol. Chem.276(34), 32373–32381 (2001). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- S. R. Leslie, A. P. Fields, and A. E. Cohen, “Convex lens-induced confinement for imaging single molecules,” Anal. Chem.82(14), 6224–6229 (2010). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- R. Littlefield and V. M. Fowler, “A minor actin catastrophe,” Nat. Cell Biol.4(9), E209–E211 (2002). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- E. Boukobza, A. Sonnenfeld, and G. Haran, “Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy,” J. Phys. Chem. B105(48), 12165–12170 (2001). [CrossRef]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- D. T. Chiu, R. M. Lorenz, and G. D. M. Jeffries, “Droplets for ultrasmall-volume analysis,” Anal. Chem.81(13), 5111–5118 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. J. Benítez, A. M. Keller, and P. Chen, “Nanovesicle trapping for studying weak protein interactions by single-molecule FRET,” Methods Enzymol.472, 41–60 (2010). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. R. Leslie, A. P. Fields, and A. E. Cohen, “Convex lens-induced confinement for imaging single molecules,” Anal. Chem.82(14), 6224–6229 (2010). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- R. Littlefield and V. M. Fowler, “A minor actin catastrophe,” Nat. Cell Biol.4(9), E209–E211 (2002). [CrossRef] [PubMed]
- D. T. Chiu, R. M. Lorenz, and G. D. M. Jeffries, “Droplets for ultrasmall-volume analysis,” Anal. Chem.81(13), 5111–5118 (2009). [CrossRef] [PubMed]
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- J. A. Theriot and T. J. Mitchison, “The nucleation-release model of actin filament dynamics in cell motility,” Trends Cell Biol.2(8), 219–222 (1992). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- E. M. De La Cruz, E. M. Ostap, and H. L. Sweeney, “Kinetic mechanism and regulation of myosin VI,” J. Biol. Chem.276(34), 32373–32381 (2001). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- J. D. Pardee and J. A. Spudich, “Purification of muscle actin,” Methods Cell Biol.24, 271–289 (1982). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- E. Boukobza, A. Sonnenfeld, and G. Haran, “Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy,” J. Phys. Chem. B105(48), 12165–12170 (2001). [CrossRef]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- J. D. Pardee and J. A. Spudich, “Purification of muscle actin,” Methods Cell Biol.24, 271–289 (1982). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- E. M. De La Cruz, E. M. Ostap, and H. L. Sweeney, “Kinetic mechanism and regulation of myosin VI,” J. Biol. Chem.276(34), 32373–32381 (2001). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- J. A. Theriot and T. J. Mitchison, “The nucleation-release model of actin filament dynamics in cell motility,” Trends Cell Biol.2(8), 219–222 (1992). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
Anal. Chem.
- D. T. Chiu, R. M. Lorenz, and G. D. M. Jeffries, “Droplets for ultrasmall-volume analysis,” Anal. Chem.81(13), 5111–5118 (2009). [CrossRef] [PubMed]
- S. R. Leslie, A. P. Fields, and A. E. Cohen, “Convex lens-induced confinement for imaging single molecules,” Anal. Chem.82(14), 6224–6229 (2010). [CrossRef] [PubMed]
Biochem. Biophys. Res. Commun.
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
Biophys. Chem.
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
Biophys. J.
- S. M. Block, “Kinesin motor mechanics: binding, stepping, tracking, gating, and limping,” Biophys. J.92(9), 2986–2995 (2007). [CrossRef] [PubMed]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
Biosystems
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
Cell
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
EMBO J.
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
J. Am. Chem. Soc.
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
J. Biol. Chem.
- E. M. De La Cruz, E. M. Ostap, and H. L. Sweeney, “Kinetic mechanism and regulation of myosin VI,” J. Biol. Chem.276(34), 32373–32381 (2001). [CrossRef] [PubMed]
J. Mol. Biol.
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
J. Phys. Chem. B
- E. Boukobza, A. Sonnenfeld, and G. Haran, “Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy,” J. Phys. Chem. B105(48), 12165–12170 (2001). [CrossRef]
Methods Cell Biol.
- J. D. Pardee and J. A. Spudich, “Purification of muscle actin,” Methods Cell Biol.24, 271–289 (1982). [CrossRef] [PubMed]
Methods Enzymol.
- J. J. Benítez, A. M. Keller, and P. Chen, “Nanovesicle trapping for studying weak protein interactions by single-molecule FRET,” Methods Enzymol.472, 41–60 (2010). [CrossRef] [PubMed]
Microbiol. Mol. Biol. Rev.
- R. Carballido-López, “The bacterial actin-like cytoskeleton,” Microbiol. Mol. Biol. Rev.70(4), 888–909 (2006). [CrossRef] [PubMed]
Nat. Cell Biol.
- R. Littlefield and V. M. Fowler, “A minor actin catastrophe,” Nat. Cell Biol.4(9), E209–E211 (2002). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
Nat. Methods
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
Nat. Struct. Mol. Biol.
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
Nature
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
Opt. Express
- J. Wenger, P.-F. Lenne, E. Popov, H. Rigneault, J. Dintinger, and T. Ebbesen, “Single molecule fluorescence in rectangular nano-apertures,” Opt. Express13(18), 7035–7044 (2005). [CrossRef] [PubMed]
Proc. Natl. Acad. Sci. U.S.A.
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
Science
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
Trends Cell Biol.
- J. A. Theriot and T. J. Mitchison, “The nucleation-release model of actin filament dynamics in cell motility,” Trends Cell Biol.2(8), 219–222 (1992). [CrossRef] [PubMed]
2012, Loveland, Nat. Methods
- A. B. Loveland, S. Habuchi, J. C. Walter, and A. M. van Oijen, “A general approach to break the concentration barrier in single-molecule imaging,” Nat. Methods9(10), 987–992 (2012). [CrossRef] [PubMed]
- W. Qiu, N. D. Derr, B. S. Goodman, E. Villa, D. Wu, W. Shih, and S. L. Reck-Peterson, “Dynein achieves processive motion using both stochastic and coordinated stepping,” Nat. Struct. Mol. Biol.19(2), 193–200 (2012). [CrossRef] [PubMed]
- M. A. DeWitt, A. Y. Chang, P. A. Combs, and A. Yildiz, “Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains,” Science335(6065), 221–225 (2012). [CrossRef] [PubMed]
- M. W. Elting, Z. Bryant, J. C. Liao, and J. A. Spudich, “Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI,” Biophys. J.100(2), 430–439 (2011). [CrossRef] [PubMed]
- A. R. Dunn, P. Chuan, Z. Bryant, and J. A. Spudich, “Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing,” Proc. Natl. Acad. Sci. U.S.A.107(17), 7746–7750 (2010). [CrossRef] [PubMed]
- S. Uemura, C. E. Aitken, J. Korlach, B. A. Flusberg, S. W. Turner, and J. D. Puglisi, “Real-time tRNA transit on single translating ribosomes at codon resolution,” Nature464(7291), 1012–1017 (2010). [CrossRef] [PubMed]
- S. R. Leslie, A. P. Fields, and A. E. Cohen, “Convex lens-induced confinement for imaging single molecules,” Anal. Chem.82(14), 6224–6229 (2010). [CrossRef] [PubMed]
- J. J. Benítez, A. M. Keller, and P. Chen, “Nanovesicle trapping for studying weak protein interactions by single-molecule FRET,” Methods Enzymol.472, 41–60 (2010). [CrossRef] [PubMed]
- B. Okumus, S. Arslan, S. M. Fengler, S. Myong, and T. Ha, “Single molecule nanocontainers made porous using a bacterial toxin,” J. Am. Chem. Soc.131(41), 14844–14849 (2009). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor,” Biophys. J.96(1), L04–L06 (2009). [CrossRef] [PubMed]
- D. T. Chiu, R. M. Lorenz, and G. D. M. Jeffries, “Droplets for ultrasmall-volume analysis,” Anal. Chem.81(13), 5111–5118 (2009). [CrossRef] [PubMed]
- J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, A. Bibillo, K. Bjornson, B. Chaudhuri, F. Christians, R. Cicero, S. Clark, R. Dalal, A. Dewinter, J. Dixon, M. Foquet, A. Gaertner, P. Hardenbol, C. Heiner, K. Hester, D. Holden, G. Kearns, X. Kong, R. Kuse, Y. Lacroix, S. Lin, P. Lundquist, C. Ma, P. Marks, M. Maxham, D. Murphy, I. Park, T. Pham, M. Phillips, J. Roy, R. Sebra, G. Shen, J. Sorenson, A. Tomaney, K. Travers, M. Trulson, J. Vieceli, J. Wegener, D. Wu, A. Yang, D. Zaccarin, P. Zhao, F. Zhong, J. Korlach, and S. Turner, “Real-time DNA sequencing from single polymerase molecules,” Science323(5910), 133–138 (2009). [CrossRef] [PubMed]
- J.-C. Liao, M. W. Elting, S. L. Delp, J. A. Spudich, and Z. Bryant, “Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity,” J. Mol. Biol.392(4), 862–867 (2009). [CrossRef] [PubMed]
- J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner, “Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures,” Proc. Natl. Acad. Sci. U.S.A.105(4), 1176–1181 (2008). [CrossRef] [PubMed]
- T. Komori, S. Nishikawa, T. Ariga, A. H. Iwane, and T. Yanagida, “Measurement system for simultaneous observation of myosin V chemical and mechanical events,” Biosystems93(1-2), 48–57 (2008). [CrossRef] [PubMed]
- T. Sakamoto, M. R. Webb, E. Forgacs, H. D. White, and J. R. Sellers, “Direct observation of the mechanochemical coupling in myosin Va during processive movement,” Nature455(7209), 128–132 (2008). [CrossRef] [PubMed]
- Y. Oguchi, S. V. Mikhailenko, T. Ohki, A. O. Olivares, E. M. De La Cruz, and S. Ishiwata, “Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function,” Proc. Natl. Acad. Sci. U.S.A.105(22), 7714–7719 (2008). [CrossRef] [PubMed]
- S. M. Block, “Kinesin motor mechanics: binding, stepping, tracking, gating, and limping,” Biophys. J.92(9), 2986–2995 (2007). [CrossRef] [PubMed]
- K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, and K. Kinosita., “Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation,” Cell130(2), 309–321 (2007). [CrossRef] [PubMed]
- H. L. Sweeney, H. Park, A. B. Zong, Z. Yang, P. R. Selvin, and S. S. Rosenfeld, “How myosin VI coordinates its heads during processive movement,” EMBO J.26(11), 2682–2692 (2007). [CrossRef] [PubMed]
- R. Carballido-López, “The bacterial actin-like cytoskeleton,” Microbiol. Mol. Biol. Rev.70(4), 888–909 (2006). [CrossRef] [PubMed]
- L. S. Churchman, Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich, “Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time,” Proc. Natl. Acad. Sci. U.S.A.102(5), 1419–1423 (2005). [CrossRef] [PubMed]
- M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb, “Zero-mode waveguides for single-molecule analysis at high concentrations,” Science299(5607), 682–686 (2003). [CrossRef] [PubMed]
- R. Littlefield and V. M. Fowler, “A minor actin catastrophe,” Nat. Cell Biol.4(9), E209–E211 (2002). [CrossRef] [PubMed]
- I. Fujiwara, S. Takahashi, H. Tadakuma, T. Funatsu, and S. Ishiwata, “Microscopic analysis of polymerization dynamics with individual actin filaments,” Nat. Cell Biol.4(9), 666–673 (2002). [CrossRef] [PubMed]
- E. Boukobza, A. Sonnenfeld, and G. Haran, “Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy,” J. Phys. Chem. B105(48), 12165–12170 (2001). [CrossRef]
- E. M. De La Cruz, E. M. Ostap, and H. L. Sweeney, “Kinetic mechanism and regulation of myosin VI,” J. Biol. Chem.276(34), 32373–32381 (2001). [CrossRef] [PubMed]
- M. Rief, R. S. Rock, A. D. Mehta, M. S. Mooseker, R. E. Cheney, and J. A. Spudich, “Myosin-V stepping kinetics: a molecular model for processivity,” Proc. Natl. Acad. Sci. U.S.A.97(17), 9482–9486 (2000). [CrossRef] [PubMed]
- K. Oiwa, J. F. Eccleston, M. Anson, M. Kikumoto, C. T. Davis, G. P. Reid, M. A. Ferenczi, J. E. Corrie, A. Yamada, H. Nakayama, and D. R. Trentham, “Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives,” Biophys. J.78(6), 3048–3071 (2000). [CrossRef] [PubMed]
- A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida, “Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin,” Cell92(2), 161–171 (1998). [CrossRef] [PubMed]
- M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, and T. Yanagida, “Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy,” Biochem. Biophys. Res. Commun.235(1), 47–53 (1997). [CrossRef] [PubMed]
- T. Funatsu, Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. D. Vale, and T. Yanagida, “Imaging and nano-manipulation of single biomolecules,” Biophys. Chem.68(1-3), 63–72 (1997). [CrossRef] [PubMed]
- J. A. Theriot and T. J. Mitchison, “The nucleation-release model of actin filament dynamics in cell motility,” Trends Cell Biol.2(8), 219–222 (1992). [CrossRef] [PubMed]
- J. D. Pardee and J. A. Spudich, “Purification of muscle actin,” Methods Cell Biol.24, 271–289 (1982). [CrossRef] [PubMed]
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