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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 6 — Jun. 27, 2013

Full field vertical scanning in short coherence digital holographic microscope

Zahra Monemhaghdoust, Frederic Montfort, Etienne Cuche, Yves Emery, Christian Depeursinge, and Christophe Moser  »View Author Affiliations

Optics Express, Vol. 21, Issue 10, pp. 12643-12650 (2013)

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In Digital holography Microscopes (DHM) implemented in the so-called “off axis” configuration, the object and reference wave fronts are not co-planar but form an angle of a few degrees. This results into two main drawbacks. First, the contrast of the interference is not uniform spatially when the light source has low coherence. The interference contrast is optimal along a line, but decreases when moving away from it, resulting in a lower image quality. Second, the non-coplanarity between the coherence plane of both wavefronts impacts the coherence vertical scanning measurement mode: when the optical path difference between the signal and the reference beam is changed, the region of maximum interference contrast shifts laterally in the plane of the objective. This results in more complex calculations to extract the topography of the sample and requires scanning over a much larger vertical range, leading to a longer measurement time. We have previously shown that by placing a volume diffractive optical element (VDOE) in the reference arm, the wavefront can be made coplanar with the object wavefront and the image plane of the microscope objective, resulting in a uniform and optimal interferogram. In this paper, we demonstrate a vertical scanning speed improvement by an order of magnitude. Noise in the phase and intensity images caused by scattering and non-uniform diffraction in the VDOE is analyzed quantitatively. Five VDOEs were fabricated with an identical procedure. We observe that VDOEs introduce a small intensity non-uniformity in the reference beam which results in a 20% noise increase in the extracted phase image as compared to the noise in extracted phase image when the VDOE is removed. However, the VDOE has no impact on the temporal noise measured from extracted phase images.

© 2013 OSA

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: April 17, 2013
Manuscript Accepted: May 2, 2013
Published: May 15, 2013

Virtual Issues
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

Zahra Monemhaghdoust, Frederic Montfort, Etienne Cuche, Yves Emery, Christian Depeursinge, and Christophe Moser, "Full field vertical scanning in short coherence digital holographic microscope," Opt. Express 21, 12643-12650 (2013)

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