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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 7 — Aug. 1, 2013

Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran

Megan L. LeBlanc, Travis R. Merritt, Jameel McMillan, James H. Westwood, and Giti A. Khodaparast  »View Author Affiliations


Optics Express, Vol. 21, Issue 12, pp. 14662-14673 (2013)
http://dx.doi.org/10.1364/OE.21.014662


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Abstract

A plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation.

© 2013 OSA

OCIS Codes
(040.3060) Detectors : Infrared
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 11, 2013
Revised Manuscript: May 29, 2013
Manuscript Accepted: May 31, 2013
Published: June 13, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Megan L. LeBlanc, Travis R. Merritt, Jameel McMillan, James H. Westwood, and Giti A. Khodaparast, "Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran," Opt. Express 21, 14662-14673 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-12-14662


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