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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 7 — Aug. 1, 2013

Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope

Tomáš Slabý, Pavel Kolman, Zbyněk Dostál, Martin Antoš, Martin Lošťák, and Radim Chmelík  »View Author Affiliations

Optics Express, Vol. 21, Issue 12, pp. 14747-14762 (2013)

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Coherence-controlled holographic microscope (CCHM) combines off-axis holography and an achromatic grating interferometer allowing for the use of light sources of arbitrary degree of temporal and spatial coherence. This results in coherence gating and strong suppression of coherent noise and parasitic interferences enabling CCHM to reach high phase measurement accuracy and imaging quality. The achievable lateral resolution reaches performance of conventional widefield microscopes, which allows resolving up to twice smaller details when compared to typical off-axis setups. Imaging characteristics can be controlled arbitrarily by coherence between two extremes: fully coherent holography and confocal-like incoherent holography. The basic setup parameters are derived and described in detail and experimental validations of imaging characteristics are demonstrated.

© 2013 OSA

OCIS Codes
(090.0090) Holography : Holography
(110.4980) Imaging systems : Partial coherence in imaging
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.3170) Microscopy : Interference microscopy
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:

Original Manuscript: December 5, 2012
Revised Manuscript: February 3, 2013
Manuscript Accepted: May 2, 2013
Published: June 13, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Tomáš Slabý, Pavel Kolman, Zbyněk Dostál, Martin Antoš, Martin Lošťák, and Radim Chmelík, "Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope," Opt. Express 21, 14747-14762 (2013)

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