OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern

A.V. Martin, A.J. Morgan, T. Ekeberg, N.D. Loh, F.R.N.C. Maia, F. Wang, J.C.H. Spence, and H.N. Chapman  »View Author Affiliations


Optics Express, Vol. 21, Issue 13, pp. 15102-15112 (2013)
http://dx.doi.org/10.1364/OE.21.015102


View Full Text Article

Enhanced HTML    Acrobat PDF (3956 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The structures of biological molecules may soon be determined with X-ray free-electron lasers without crystallization by recording the coherent diffraction patterns of many identical copies of a molecule. Most analysis methods require a measurement of each molecule individually. However, current injection methods deliver particles to the X-ray beam stochastically and the maximum yield of single particle measurements is 37% at optimal concentration. The remaining 63% of pulses intercept no particles or multiple particles. We demonstrate that in the latter case single particle diffraction patterns can be extracted provided the particles are sufficiently separated. The technique has the potential to greatly increase the amount of data available for three-dimensional imaging of identical particles with X-ray lasers.

© 2013 OSA

OCIS Codes
(110.7440) Imaging systems : X-ray imaging
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Imaging Systems

History
Original Manuscript: April 24, 2013
Revised Manuscript: June 7, 2013
Manuscript Accepted: June 8, 2013
Published: June 17, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Citation
A.V. Martin, A.J. Morgan, T. Ekeberg, N.D. Loh, F.R.N.C. Maia, F. Wang, J.C.H. Spence, and H.N. Chapman, "The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern," Opt. Express 21, 15102-15112 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-13-15102


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. V. Ayvazyan, N. Baboi, J. Bähr, V. Balandin, B. Beutner, A. Brandt, I. Bohnet, A. Bolzmann, R. Brinkmann, and O. I. Brovko, “First operation of a free-electron laser generating gw power radiation at 32 nm wavelength,” Eur. Phys. J. D37, 297–303 (2006). [CrossRef]
  2. P. Emma, R. Akre, J. Arthur, R. M. Bionta, C. Bostedt, J. Bozek, A. Brachmann, P. H. Bucksbaum, R. Coffee, F. J. Decker, Y. Ding, D. Dowell, S. Edstrom, A. Fisher, J. Frisch, S. Gilevich, J. Hastings, G. Hays, P. Hering, Z. Huang, R. Iverson, H. Loos, M. Messerschmidt, A. Miahnahri, S. Moeller, H. -D. Nuhn, D. Pile, D. Ratner, J. Rzepiela, D. Schultz, T. Smith, P. Stefan, H. Tompkins, J. Turner, J. Welch, W. White, J. Wu, G. Yocky, and J. N. Galayda, “First lasing and operation of an angstrom-wavelength free-electron laser,” Nat. Photon.4, 641 (2010). [CrossRef]
  3. N. G. A. Abrescia, D. H. Bamford, J. M. Grimes, and D. I. Stuart, “Structure unifies the viral universe,” Annu. Rev. Biochem.81, 795–822 (2012). [CrossRef] [PubMed]
  4. E. P. Carpenter, K. Beis, A. D. Cameron, and S. Iwata, “Overcoming the challenges of membrane protein crystallography,” Current Opinion in Structural Biology18, 581–586 (2008). [CrossRef] [PubMed]
  5. G. Huldt, A. Szoke, and J. Hajdu, “Diffraction imaging of single particles and biomolecules,” J. Struct. Biol.144, 219–227 (2003). [CrossRef] [PubMed]
  6. D. P. DePonte, U. Weierstall, K. Schmidt, J. Warner, D. Starodub, J. C. H. Spence, and R. B. Doak, “Gas dynamic virtual nozzle for generation of microscopic droplet streams,” J. Phys. D: Appl. Phys.41, 195505 (2008). [CrossRef]
  7. M. J. Bogan, S. Boutet, H. N. Chapman, S. Marchesini, A. Barty, W. H. Benner, U. Rohner, M. Frank, S. P. Hau-Riege, S. Bajt, B. W. Woods, M. M. Seibert, B. Iwan, N. Timneanu, J. Hajdu, and J. Schulz, “Aerosol imaging with a soft x-ray free electron laser,” Aerosol Sci. Tech.44, i–vi(2010). [CrossRef]
  8. N. D. Loh and V. Elser, “Reconstruction algorithm for single-particle diffraction imaging experiments,” Phys. Rev. E80, 026705 (2009). [CrossRef]
  9. R. Fung, V. Shneerson, D. K. Saldin, and A. Ourmazd, “Structure from fleeting illumination of faint spinning objects in flight,” Nat. Phys.5, 64–67 (2009). [CrossRef]
  10. G. Bortel and M. Tegze, “Common arc method for diffraction pattern orientation,” Acta Crystallogr.A67, 533–543 (2011).
  11. D. Starodub, A. Aquila, S. Bajt, M. Barthelmess, A. Barty, C. Bostedt, J. D. Bozek, N. Coppola, R. B. Doak, S. W. Epp, B. Erk, L. Foucar, L. Gumprecht, C. Y. Hampton, A. Hartmann, R. Hartmann, P. Holl, S. Kassemeyer, N. Kimmel, H. Laksmono, M. Liang, N. D. Loh, L. Lomb, A. V. Martin, K. Nass, C. Reich, D. Rolles, B. Rudek, A. Rudenko, J. Schulz, R. L. Shoeman, R. G. Sierra, H. Soltau, J. Steinbrener, F. Stellato, S. Stern, G. Weidenspointner, M. Frank, J. Ullrich, L. Strüder, I. Schlichting, H. N. Chapman, J. C. H. Spence, and M. J. Bogan, “Single-particle structure determination by correlations of snapshot x-ray diffraction patterns,” Nat. Commun.3, 1276 (2012). [CrossRef] [PubMed]
  12. M. J. Buerger, Vector Space and its Application in Crystal-Structure Investigation(Wiley, New York, 1959).
  13. H. He, S. Marchesini, M. Howells, U. Weierstall, G. Hembree, and J. C. H. Spence, “Experimental lensless soft-x-ray imaging using iterative algorithms: phasing diffuse scattering,” Acta Crystallogr.A59, 143–152 (2003).
  14. X. Huang, J. Nelson, J. Steinbrener, J. Kirz, J. Turner, and C. Jacobsen, “Incorrect support and missing center tolerances of phasing algorithms.” Opt. Express18, 26441–26449 (2010). [CrossRef] [PubMed]
  15. P. Thibault, P. Elser, C. Jacobsen, D. Shapiro, and D. Sayre, “Reconstruction of a yeast cell from x-ray diffraction data,” Acta Crystallogr.A62, 248–261 (2006).
  16. W. H. Press, B. P. Flannery, S. A. Teukolsky, and W. T. Vetterling, Numerical Recipes: The Art of Scientific Computing(Cambridge University Press, Cambridge, 1986).
  17. A. Martin, N. Loh, C. Hampton, R. Sierra, F. Wang, A. Aquila, S. Bajt, M. Barthelmess, C. Bostedt, J. Bozek, N. Coppola, S. Epp, B. Erk, H. Fleckenstein, L. Foucar, M. Frank, H. Graafsma, L. Gumprecht, A. Hartmann, R. Hartmann, G. Hauser, H. Hirsemann, P. Holl, S. Kassemeyer, N. Kimmel, M. Liang, L. Lomb, F. Maia, S. Marchesini, K. Nass, E. Pedersoli, C. Reich, D. Rolles, B. Rudek, A. Rudenko, J. Schulz, R. Shoeman, H. Soltau, D. Starodub, J. Steinbrener, F. Stellato, L. Strüder, J. Ullrich, G. Weidenspointner, T. White, C. Wunderer, A. Barty, I. Schlichting, M. Bogan, and H. Chapman, “Femtosecond dark-field imaging with an x-ray free electron laser,” Opt. Express20, 13501–13512 (2012). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited