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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

Near shot-noise limited hyperspectral stimulated Raman scattering spectroscopy using low energy lasers and a fast CMOS array

William Rock, Mischa Bonn, and Sapun H. Parekh  »View Author Affiliations

Optics Express, Vol. 21, Issue 13, pp. 15113-15120 (2013)

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We demonstrate near shot-noise limited hyperspectral stimulated Raman scattering (SRS) spectroscopy using oscillator-only excitation conditions. Using a fast CMOS camera synchronized to an acousto-optic modulator and subtracting subsequent frames acquired at up to 1 MHz frame rates, we demonstrate demodulation and recovery of the SRS spectrum. Surprisingly, we observe that the signal-to-noise of SRS spectra is invariant at modulation frequencies down to 2.5 kHz. Our approach allows for a direct comparison of SRS with coherent anti-Stokes Raman scattering (CARS) spectroscopy under identical experimental conditions. Our findings suggest that hyperspectral SRS imaging with shot-noise limited performance at biologically compatible excitation energies is feasible after minor modifications to fast frame-rate CMOS array technology.

© 2013 OSA

OCIS Codes
(040.1240) Detectors : Arrays
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6290) Spectroscopy : Spectroscopy, four-wave mixing
(180.5655) Microscopy : Raman microscopy

ToC Category:

Original Manuscript: April 25, 2013
Revised Manuscript: June 6, 2013
Manuscript Accepted: June 6, 2013
Published: June 17, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

William Rock, Mischa Bonn, and Sapun H. Parekh, "Near shot-noise limited hyperspectral stimulated Raman scattering spectroscopy using low energy lasers and a fast CMOS array," Opt. Express 21, 15113-15120 (2013)

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