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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

A multi-modal stereo microscope based on a spatial light modulator

M. P. Lee, G. M. Gibson, R. Bowman, S. Bernet, M. Ritsch-Marte, D. B. Phillips, and M. J. Padgett  »View Author Affiliations


Optics Express, Vol. 21, Issue 14, pp. 16541-16551 (2013)
http://dx.doi.org/10.1364/OE.21.016541


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Abstract

Spatial Light Modulators (SLMs) can emulate the classic microscopy techniques, including differential interference (DIC) contrast and (spiral) phase contrast. Their programmability entails the benefit of flexibility or the option to multiplex images, for single-shot quantitative imaging or for simultaneous multi-plane imaging (depth-of-field multiplexing). We report the development of a microscope sharing many of the previously demonstrated capabilities, within a holographic implementation of a stereo microscope. Furthermore, we use the SLM to combine stereo microscopy with a refocusing filter and with a darkfield filter. The instrument is built around a custom inverted microscope and equipped with an SLM which gives various imaging modes laterally displaced on the same camera chip. In addition, there is a wide angle camera for visualisation of a larger region of the sample.

© 2013 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(230.3720) Optical devices : Liquid-crystal devices

ToC Category:
Imaging Systems

History
Original Manuscript: May 6, 2013
Revised Manuscript: June 11, 2013
Manuscript Accepted: June 14, 2013
Published: July 2, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Citation
M. P. Lee, G. M. Gibson, R. Bowman, S. Bernet, M. Ritsch-Marte, D. B. Phillips, and M. J. Padgett, "A multi-modal stereo microscope based on a spatial light modulator," Opt. Express 21, 16541-16551 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-14-16541


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