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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

Swept source optical coherence microscopy using a 1310 nm VCSEL light source

Osman O. Ahsen, Yuankai K. Tao, Benjamin M. Potsaid, Yuri Sheikine, James Jiang, Ireneusz Grulkowski, Tsung-Han Tsai, Vijaysekhar Jayaraman, Martin F. Kraus, James L. Connolly, Joachim Hornegger, Alex Cable, and James G. Fujimoto  »View Author Affiliations


Optics Express, Vol. 21, Issue 15, pp. 18021-18033 (2013)
http://dx.doi.org/10.1364/OE.21.018021


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Abstract

We demonstrate high speed, swept source optical coherence microscopy (OCM) using a MEMS tunable vertical cavity surface-emitting laser (VCSEL) light source. The light source had a sweep rate of 280 kHz, providing a bidirectional axial scan rate of 560 kHz. The sweep bandwidth was 117 nm centered at 1310 nm, corresponding to an axial resolution of 13.1 µm in air, corresponding to 8.1 µm (9.6 µm spectrally shaped) in tissue. Dispersion mismatch from different objectives was compensated numerically, enabling magnification and field of view to be easily changed. OCM images were acquired with transverse resolutions between 0.86 µm - 3.42 µm using interchangeable 40X, 20X and 10X objectives with ~600 µm x 600 µm, ~1 mm x 1 mm and ~2 mm x 2 mm field-of-view (FOV), respectively. Parasitic variations in path length with beam scanning were corrected numerically. These features enable swept source OCM to be integrated with a wide range of existing scanning microscopes. Large FOV mosaics were generated by serially acquiring adjacent overlapping microscopic fields and combining them in post-processing. Fresh human colon, thyroid and kidney specimens were imaged ex vivo and compared to matching histology sections, demonstrating the ability of OCM to image tissue specimens.

© 2013 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(140.3600) Lasers and laser optics : Lasers, tunable
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 25, 2013
Revised Manuscript: July 8, 2013
Manuscript Accepted: July 12, 2013
Published: July 19, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Osman O. Ahsen, Yuankai K. Tao, Benjamin M. Potsaid, Yuri Sheikine, James Jiang, Ireneusz Grulkowski, Tsung-Han Tsai, Vijaysekhar Jayaraman, Martin F. Kraus, James L. Connolly, Joachim Hornegger, Alex Cable, and James G. Fujimoto, "Swept source optical coherence microscopy using a 1310 nm VCSEL light source," Opt. Express 21, 18021-18033 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-15-18021


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