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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 9 — Oct. 2, 2013

Dynamic light scattering microscope: Accessing opaque samples with high spatial resolution

Takashi Hiroi and Mitsuhiro Shibayama  »View Author Affiliations


Optics Express, Vol. 21, Issue 17, pp. 20260-20267 (2013)
http://dx.doi.org/10.1364/OE.21.020260


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Abstract

We developed a new technique that conducts dynamic light scattering (DLS) under a microscope with high spatial resolution. This technique dramatically extends the range of DLS application from transparent to opaque samples. The total scattered electric field contains both electric field generated from the samples and time-independent reflected electric field. These two components are decomposed by applying a partial heterodyne method. By using this technique, we successfully calculate the characteristic size distribution of both multiple-scattering samples and strong light-absorbing samples. This is the first study to observe the collective motion of particles in a highly concentrated solution by using DLS.

© 2013 OSA

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(290.1350) Scattering : Backscattering
(290.4210) Scattering : Multiple scattering
(300.6310) Spectroscopy : Spectroscopy, heterodyne

ToC Category:
Microscopy

History
Original Manuscript: April 22, 2013
Revised Manuscript: July 21, 2013
Manuscript Accepted: August 7, 2013
Published: August 22, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Takashi Hiroi and Mitsuhiro Shibayama, "Dynamic light scattering microscope: Accessing opaque samples with high spatial resolution," Opt. Express 21, 20260-20267 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-17-20260


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