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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 10 — Nov. 8, 2013

High efficiency ring-lens supercritical angle fluorescence (SAF) detection for optimum bioassay performance

Dirk Kurzbuch, Martin Somers, and Colette McDonagh  »View Author Affiliations


Optics Express, Vol. 21, Issue 19, pp. 22070-22075 (2013)
http://dx.doi.org/10.1364/OE.21.022070


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Abstract

We present a polymer biochip with embedded optics which allows the detection of supercritical angle fluorescence (SAF) without losses due to total internal reflection within the substrate. The chip design comprises structured spherical and aspherical optical elements on the bottom, while the top is chemically functionalized for direct binding of biomolecules. Furthermore, this design facilitates integration in lab-on-a-chip systems with appropriate microfluidics. In the confocal optical setup an ellipsoidal mirror is used for collection of SAF light above the critical angle of the water-polymer interface, which is detected by a photon-counting detector. The work presented here represents a proof of concept for performing sensitive and rapid point-of-care testing, using this low-cost, robust and disposable optical biochip platform. The performance of the platform was validated using direct binding DNA and human IgG assays which yielded low limits of detection 10pM for DNA and 10pg/ml for human IgG.

© 2013 OSA

OCIS Codes
(080.2740) Geometric optics : Geometric optical design
(180.1790) Microscopy : Confocal microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(080.4035) Geometric optics : Mirror system design

ToC Category:
Microscopy

History
Original Manuscript: April 18, 2013
Revised Manuscript: August 2, 2013
Manuscript Accepted: August 13, 2013
Published: September 12, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Dirk Kurzbuch, Martin Somers, and Colette McDonagh, "High efficiency ring-lens supercritical angle fluorescence (SAF) detection for optimum bioassay performance," Opt. Express 21, 22070-22075 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-19-22070


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