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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 2 — Mar. 4, 2013

The wide-field optical sectioning of microlens array and structured illumination-based plane-projection multiphoton microscopy

Jiun-Yann Yu, Daniel B. Holland, Geoffrey A. Blake, and Chin-Lin Guo  »View Author Affiliations


Optics Express, Vol. 21, Issue 2, pp. 2097-2109 (2013)
http://dx.doi.org/10.1364/OE.21.002097


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Abstract

We present a theoretical investigation of an optical microscope design that achieves wide-field, multiphoton fluorescence microscopy with finer axial resolution than confocal microscopy. Our technique creates a thin plane of excitation light at the sample using height-staggered microlens arrays (HSMAs), wherein the height staggering of microlenses generate temporal focusing to suppress out-of-focus excitation, and the dense spacing of microlenses enables the implementation of structured illumination technique to eliminate residual out-of-focus signal. We use physical optics-based numerical simulations to demonstrate that our proposed technique can achieve diffraction-limited three-dimensional imaging through a simple optical design.

© 2013 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.6900) Microscopy : Three-dimensional microscopy
(260.1960) Physical optics : Diffraction theory
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 31, 2012
Revised Manuscript: December 23, 2012
Manuscript Accepted: December 26, 2012
Published: January 18, 2013

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Jiun-Yann Yu, Daniel B. Holland, Geoffrey A. Blake, and Chin-Lin Guo, "The wide-field optical sectioning of microlens array and structured illumination-based plane-projection multiphoton microscopy," Opt. Express 21, 2097-2109 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-2-2097


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