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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Line temporal focusing characteristics in transparent and scattering media

Hod Dana, Nimrod Kruger, Aviv Ellman, and Shy Shoham  »View Author Affiliations


Optics Express, Vol. 21, Issue 5, pp. 5677-5687 (2013)
http://dx.doi.org/10.1364/OE.21.005677


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Abstract

Line illumination geometries have advantageous properties for temporal focusing nonlinear microscopy. The characteristics of line temporal focusing (LITEF) in transparent and scattering media are studied here both experimentally and using numerical model simulations. We introduce an approximate analytical formula for the dependence of axial sectioning on the laser and microscope's parameters. Furthermore, we show that LITEF is more robust to tissue scattering than wide-field temporal focusing, and can penetrate much deeper into scattering tissue while maintaining good sectioning capabilities. Based on these observations, we propose a new design for LITEF-based tissue imaging at depths that could potentially exceed the out-of-focus physical excitation limit.

© 2013 OSA

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(290.5850) Scattering : Scattering, particles

ToC Category:
Microscopy

History
Original Manuscript: October 17, 2012
Revised Manuscript: December 26, 2012
Manuscript Accepted: January 25, 2013
Published: March 1, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Hod Dana, Nimrod Kruger, Aviv Ellman, and Shy Shoham, "Line temporal focusing characteristics in transparent and scattering media," Opt. Express 21, 5677-5687 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-5-5677


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