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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Separation of overlapped particles in digital holographic microscopy

Ahmed El Mallahi and Frank Dubois  »View Author Affiliations


Optics Express, Vol. 21, Issue 5, pp. 6466-6479 (2013)
http://dx.doi.org/10.1364/OE.21.006466


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Abstract

In this paper, we present a procedure to separate aggregates of overlapped particles in digital holograms, based on a focus plane analysis applied to each particle. The method can be applied either on phase or on amplitude objects, according that each object has a border in one focus plane. Numerical simulations are performed to quantify the robustness of the process by increasing the overlapping areas between the particles. The separation algorithm is successfully demonstrated experimentally on different types of aggregates.

© 2013 OSA

OCIS Codes
(090.0090) Holography : Holography
(100.0100) Image processing : Image processing
(100.2000) Image processing : Digital image processing
(100.6890) Image processing : Three-dimensional image processing
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: February 22, 2013
Revised Manuscript: February 27, 2013
Manuscript Accepted: February 27, 2013
Published: March 7, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Ahmed El Mallahi and Frank Dubois, "Separation of overlapped particles in digital holographic microscopy," Opt. Express 21, 6466-6479 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-5-6466


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