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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 4 — May. 22, 2013

Differential interferometric particle tracking on the subnanometer- and submillisecond-scale

Dennis Müller, Dieter R. Klopfenstein, and Rainer G. Ulbrich  »View Author Affiliations


Optics Express, Vol. 21, Issue 6, pp. 7362-7372 (2013)
http://dx.doi.org/10.1364/OE.21.007362


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Abstract

We describe an interferometric method to measure the movement of a subwavelength probe particle relative to an immobilized reference particle with high spatial (Δx = 0.9nm) and temporal (Δt = 200μs) resolution. The differential method eliminates microscope stage drift. An upright microscope is equipped with laser dark field illumination (λ0 = 532nm, P0 = 30mW) and a compact modified Mach-Zehnder interferometer is mounted on the camera exit of the microscope, where the beams of scattered light of both particles are combined. The resulting interferograms provide in two channels subnanometer information about the motion of the probe particle relative to the reference particle. The interferograms are probed with two avalanche photodiodes. We applied this method to measuring the movement of kinesin along microtubules and were able to resolve the generic 8-nm steps at high ATP concentrations without external forces.

© 2013 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: December 19, 2012
Revised Manuscript: February 15, 2013
Manuscript Accepted: February 21, 2013
Published: March 18, 2013

Virtual Issues
Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Dennis Müller, Dieter R. Klopfenstein, and Rainer G. Ulbrich, "Differential interferometric particle tracking on the subnanometer- and submillisecond-scale," Opt. Express 21, 7362-7372 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-6-7362


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References

  1. S. Kamimura, “Direct measurement of nanometric displacement under an optical microscope,” Appl. Opt.26, 3425–3427 (1987). [CrossRef] [PubMed]
  2. W. Denk and W. W. Webb, “Optical measurement of picometer displacements of transparent microscopic objects,” Appl. Opt.29, 2382–2391 (1990). [CrossRef] [PubMed]
  3. R. M. Simmons, J. T. Finer, S. Chu, and J. A. Spudich, “Quantitative measurements of force and displacement using an optical trap.” Biophys. J.70, 1813–1822 (1996). [CrossRef] [PubMed]
  4. A. Yildiz, J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin, “Myosin v walks hand-overhand: single fluorophore imaging with 1.5-nm localization,” Science300, 2061–2065 (2003). [CrossRef] [PubMed]
  5. J. Gelles, B. J. Schnapp, and M. P. Sheetz, “Tracking kinesin-driven movements with nanometre-scale precision,” Nature331, 450–453 (1988). [CrossRef] [PubMed]
  6. M. Nishiyama, E. Muto, Y. Inoue, T. Yanagida, and H. Higuchi, “Substeps within the 8-nm step of the atpase cycle of single kinesin molecules,” Nat. Cell Biol.3, 425–428 (2001). [CrossRef] [PubMed]
  7. K. Svoboda, C. F. Schmidt, B. J. Schnapp, and S. M. Block, “Direct observation of kinesin stepping by optical trapping interferometry,” Nature365, 721–727 (1993). [CrossRef] [PubMed]
  8. E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, “Direct observation of base-pair stepping by rna polymerase,” Nature438, 460–465 (2005). [CrossRef] [PubMed]
  9. O. Otto, F. Czerwinski, J. L. Gornall, G. Stober, L. B. Oddershede, R. Seidel, and U. F. Keyser, “Real-time particle tracking at 10,000 fps using optical fiber illumination,” Opt. Express18, 22722–22733 (2010). [CrossRef] [PubMed]
  10. R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J.82, 2775–2783 (2002). [CrossRef] [PubMed]
  11. V. Jacobsen, P. Stoller, C. Brunner, V. Vogel, and V. Sandoghdar, “Interferometric optical detection and tracking of very small gold nanoparticles at a water-glass interface,” Opt. Express14, 405–414 (2006). [CrossRef] [PubMed]
  12. A. R. Carter, G. M. King, T. A. Ulrich, W. Halsey, D. Alchenberger, and T. T. Perkins, “Stabilization of an optical microscope to 0.1 nm in three dimensions,” Appl. Opt.46, 421–427 (2007). [CrossRef] [PubMed]
  13. M. Mahamdeh and E. Schäffer, “Optical tweezers with millikelvin precision of temperature-controlled objectives and base-pair resolution,” Opt. Express17, 17190–17199 (2009). [CrossRef] [PubMed]
  14. G. Cappello, M. Badoual, A. Ott, J. Prost, and L. Busoni, “Kinesin motion in the absence of external forces characterized by interference total internal reflection microscopy,” Phys. Rev. E68, 021907 (2003). [CrossRef]
  15. M. Born and E. Wolf, Principles of Optics (Pergamon PressNew York, 1980).
  16. Z. Wang, S. Khan, and M. P. Sheetz, “Single cytoplasmic dynein molecule movements: Characterization and comparison with kinesin,” Biophys. J.69, 2011–2023 (1995). [CrossRef] [PubMed]
  17. M. W. Allersma, F. Gittes, M. J. deCastro, R. J. Stewart, and C. F. Schmidt, “Two-dimensional tracking of ncd motility by back focal plane interferometry,” Biophys. J.74, 1074–1085 (1998). [CrossRef] [PubMed]
  18. K. Svoboda and S. M. Block, “Biological applications of optical forces,” Annu. Rev. Biophys. Biomol. Struct.23, 247–285 (1994). [CrossRef]
  19. A. J. Hunt, F. Gittes, and J. Howard, “The force exerted by a single kinesin molecule against a viscous load.” Biophys. J.67, 766–781 (1994). [CrossRef] [PubMed]

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