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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 5 — Jun. 6, 2013

Dye exclusion microfluidic microscopy

Ethan Schonbrun, Giuseppe Di Caprio, and Diane Schaak  »View Author Affiliations


Optics Express, Vol. 21, Issue 7, pp. 8793-8798 (2013)
http://dx.doi.org/10.1364/OE.21.008793


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Abstract

We present an optical system to measure height maps of non-adherent cells as they flow through a microfluidic channel. The cells are suspended in an index-matching absorbing buffer, where cell height is evaluated by measuring the difference in absorption between the cell and the background. Unlike interferometric microscopes, the measured cell height is nearly independent of the cell’s optical properties. The height maps are captured using a single exposure of a color camera, and consequently the system is capable of high-throughput characterization of large collections of cells. Using this system, we have measured more than 1600 height maps and volumes of three different leukemia cell lines.

© 2013 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: February 19, 2013
Revised Manuscript: March 20, 2013
Manuscript Accepted: March 26, 2013
Published: April 2, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Ethan Schonbrun, Giuseppe Di Caprio, and Diane Schaak, "Dye exclusion microfluidic microscopy," Opt. Express 21, 8793-8798 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-7-8793


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References

  1. A. Tzur, J. K. Moore, P. Jorgensen, H. M. Shapiro, and M. W. Kirschner, “Optimizing optical flow cytometry for cell volume-based sorting and analysis,” PLoS ONE6(1), e16053 (2011). [CrossRef] [PubMed]
  2. M. Wendel, L. Bazhenova, R. Boshuizen, A. Kolatkar, M. Honnatti, E. H. Cho, D. Marrinucci, A. Sandhu, A. Perricone, P. Thistlethwaite, K. Bethel, J. Nieva, M. van den Heuvel, and P. Kuhn, “Fluid biopsy for circulating tumor cell identification in patients with early-and late-stage non-small cell lung cancer: a glimpse into lung cancer biology,” Phys. Biol.9(1), 016005 (2012). [CrossRef] [PubMed]
  3. T. R. Jones, A. E. Carpenter, M. R. Lamprecht, J. Moffat, S. J. Silver, J. K. Grenier, A. B. Castoreno, U. S. Eggert, D. E. Root, P. Golland, and D. M. Sabatini, “Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning,” Proc. Natl. Acad. Sci. U.S.A.106(6), 1826–1831 (2009). [CrossRef] [PubMed]
  4. D. A. Basiji, W. E. Ortyn, L. Liang, V. Venkatachalam, and P. Morrissey, “Cellular image analysis and imaging by flow cytometry,” Clin. Lab. Med.27(3), 653–670, viii (2007). [CrossRef] [PubMed]
  5. E. Schonbrun, S. S. Gorthi, and D. Schaak, “Microfabricated multiple field of view imaging flow cytometry,” Lab Chip12(2), 268–273 (2011). [CrossRef] [PubMed]
  6. J. M. Higgins and L. Mahadevan, “Physiological and pathological population dynamics of circulating human red blood cells,” Proc. Natl. Acad. Sci. U.S.A.107(47), 20587–20592 (2010). [CrossRef] [PubMed]
  7. S. Son, A. Tzur, Y. Weng, P. Jorgensen, J. Kim, M. W. Kirschner, and S. R. Manalis, “Direct observation of mammalian cell growth and size regulation,” Nat. Methods9(9), 910–912 (2012). [CrossRef] [PubMed]
  8. B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A73A(10), 895–903 (2008). [CrossRef] [PubMed]
  9. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006). [CrossRef] [PubMed]
  10. B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt.47(4), A52–A61 (2008). [CrossRef] [PubMed]
  11. G. Di Caprio, M. A. Gioffre, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron.16(4), 833–840 (2010). [CrossRef]
  12. A. Greenbaum, W. Luo, T. W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods9(9), 889–895 (2012). [CrossRef] [PubMed]
  13. S. S. Gorthi and E. Schonbrun, “Phase imaging flow cytometry using a focus-stack collecting microscope,” Opt. Lett.37(4), 707–709 (2012). [CrossRef] [PubMed]
  14. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007). [CrossRef] [PubMed]
  15. M. L. Gray, R. A. Hoffman, and W. P. Hansen, “A new method for cell volume measurement based on volume exclusion of a fluorescent dye,” Cytometry3(6), 428–434 (1983). [CrossRef] [PubMed]
  16. M. A. Model, A. K. Khitrin, and J. L. Blank, “Measurement of the absorption of concentrated dyes and their use for quantitative imaging of surface topography,” J. Microsc.231(1), 156–167 (2008). [CrossRef] [PubMed]
  17. J. C. M. Antón, J. Alonso, J. A. G. Pedrero, and J. A. Quiroga, “Topographic optical profilometry by absorption in liquids,” Opt. Express20(27), 28631–28640 (2012). [CrossRef] [PubMed]
  18. R. Barer and S. Joseph, “Refractometry of living cells,” Q. J. Microsc. Sci.95, 399–423 (1954).
  19. McCormick® Yellow Food Color.
  20. J. Dai and M. P. Sheetz, “Membrane tether formation from blebbing cells,” Biophys. J.77(6), 3363–3370 (1999). [CrossRef] [PubMed]

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