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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 5 — Jun. 6, 2013

Extended depth of field microscopy for rapid volumetric two-photon imaging

Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy  »View Author Affiliations

Optics Express, Vol. 21, Issue 8, pp. 10095-10104 (2013)

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Two-photon fluorescence microscopy is an influential tool in biology, providing valuable information on the activity of cells deep inside the tissue. However, it is limited by its low speed for imaging volume samples. Here we present the design of a two-photon scanning microscope with an extended and adjustable depth of field, which improves the temporal resolution for sampling thick samples. Moreover, this method implies no loss of optical power and resolution, and can be easily integrated into most commercial laser-scanning microscopy systems. We demonstrate experimentally the gain in performance of the system by comparing volumetric scans of neuronal structures with a standard versus an extended depth of field system.

© 2013 OSA

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: January 11, 2013
Revised Manuscript: March 8, 2013
Manuscript Accepted: March 14, 2013
Published: April 16, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy, "Extended depth of field microscopy for rapid volumetric two-photon imaging," Opt. Express 21, 10095-10104 (2013)

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