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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 5 — Jun. 6, 2013

Pump-probe nonlinear phase dispersion spectroscopy

Francisco E. Robles, Prathyush Samineni, Jesse W. Wilson, and Warren S. Warren  »View Author Affiliations

Optics Express, Vol. 21, Issue 8, pp. 9353-9364 (2013)

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Pump-probe microscopy is an imaging technique that delivers molecular contrast of pigmented samples. Here, we introduce pump-probe nonlinear phase dispersion spectroscopy (PP-NLDS), a method that leverages pump-probe microscopy and spectral-domain interferometry to ascertain information from dispersive and resonant nonlinear effects. PP-NLDS extends the information content to four dimensions (phase, amplitude, wavelength, and pump-probe time-delay) that yield unique insight into a wider range of nonlinear interactions compared to conventional methods. This results in the ability to provide highly specific molecular contrast of pigmented and non-pigmented samples. A theoretical framework is described, and experimental results and simulations illustrate the potential of this method. Implications for biomedical imaging are discussed.

© 2013 OSA

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.3270) Nonlinear optics : Kerr effect
(300.6420) Spectroscopy : Spectroscopy, nonlinear
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: February 13, 2013
Revised Manuscript: April 1, 2013
Manuscript Accepted: April 4, 2013
Published: April 9, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Francisco E. Robles, Prathyush Samineni, Jesse W. Wilson, and Warren S. Warren, "Pump-probe nonlinear phase dispersion spectroscopy," Opt. Express 21, 9353-9364 (2013)

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