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Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 6 — Jun. 27, 2013

Enhancing diffractive multi-plane microscopy using colored illumination

Alexander Jesacher, Clemens Roider, and Monika Ritsch-Marte  »View Author Affiliations


Optics Express, Vol. 21, Issue 9, pp. 11150-11161 (2013)
http://dx.doi.org/10.1364/OE.21.011150


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Abstract

We present a method to increase the number of simultaneously imaged focal planes in diffractive multi-plane imaging. We exploit the chromatic properties of diffraction by using multicolor LED illumination and demonstrate time-synchronous imaging of up to 21 focal planes.We discuss the possibilities and limits given by the use of a liquid crystal spatial light modulator to display the diffractive patterns. The method is suitable for wide-field transmission and reflection microscopy.

© 2013 osa

OCIS Codes
(050.1970) Diffraction and gratings : Diffractive optics
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(230.3720) Optical devices : Liquid-crystal devices

ToC Category:
Microscopy

History
Original Manuscript: February 20, 2013
Revised Manuscript: April 11, 2013
Manuscript Accepted: April 11, 2013
Published: April 30, 2013

Virtual Issues
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Alexander Jesacher, Clemens Roider, and Monika Ritsch-Marte, "Enhancing diffractive multi-plane microscopy using colored illumination," Opt. Express 21, 11150-11161 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-21-9-11150


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