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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 3 — Mar. 6, 2014

Bispectral coding: compressive and high-quality acquisition of fluorescence and reflectance

Jinli Suo, Liheng Bian, Feng Chen, and Qionghai Dai  »View Author Affiliations

Optics Express, Vol. 22, Issue 2, pp. 1697-1712 (2014)

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Fluorescence widely coexists with reflectance in the real world, and an accurate representation of these two components in a scene is vitally important. Despite the rich knowledge of fluorescence mechanisms and behaviors, traditional fluorescence imaging approaches are quite limited in efficiency and quality. To address these two shortcomings, we propose a bispectral coding scheme to capture fluorescence and reflectance: multiplexing code is applied to excitation spectrums to raise the signal-to-noise ratio, and compressive sampling code is applied to emission spectrums for high efficiency. For computational reconstruction from the sparse coded measurements, the redundancy in both components promises recovery from sparse measurements, and the difference between their redundancies promises accurate separation. Mathematically, we cast the reconstruction as a joint optimization, whose solution can be derived by the Augmented Lagrange Method. In our experiment, results on both synthetic data and real data captured by our prototype validate the proposed approach, and we also demonstrate its advantages in two computer vision tasks—photorealistic relighting and segmentation.

© 2014 Optical Society of America

OCIS Codes
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(110.1758) Imaging systems : Computational imaging
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Imaging Systems

Original Manuscript: September 12, 2013
Revised Manuscript: December 19, 2013
Manuscript Accepted: January 5, 2014
Published: January 17, 2014

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Jinli Suo, Liheng Bian, Feng Chen, and Qionghai Dai, "Bispectral coding: compressive and high-quality acquisition of fluorescence and reflectance," Opt. Express 22, 1697-1712 (2014)

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