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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Real-Space x-ray tomographic reconstruction of randomly oriented objects with sparse data frames

Kartik Ayyer, Hugh T. Philipp, Mark W. Tate, Veit Elser, and Sol M. Gruner  »View Author Affiliations


Optics Express, Vol. 22, Issue 3, pp. 2403-2413 (2014)
http://dx.doi.org/10.1364/OE.22.002403


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Abstract

Schemes for X-ray imaging single protein molecules using new x-ray sources, like x-ray free electron lasers (XFELs), require processing many frames of data that are obtained by taking temporally short snapshots of identical molecules, each with a random and unknown orientation. Due to the small size of the molecules and short exposure times, average signal levels of much less than 1 photon/pixel/frame are expected, much too low to be processed using standard methods. One approach to process the data is to use statistical methods developed in the EMC algorithm (Loh & Elser, Phys. Rev. E, 2009) which processes the data set as a whole. In this paper we apply this method to a real-space tomographic reconstruction using sparse frames of data (below 10−2 photons/pixel/frame) obtained by performing x-ray transmission measurements of a low-contrast, randomly-oriented object. This extends the work by Philipp et al. (Optics Express, 2012) to three dimensions and is one step closer to the single molecule reconstruction problem.

© 2014 Optical Society of America

OCIS Codes
(000.2190) General : Experimental physics
(040.7480) Detectors : X-rays, soft x-rays, extreme ultraviolet (EUV)
(100.6950) Image processing : Tomographic image processing
(110.4280) Imaging systems : Noise in imaging systems
(110.7440) Imaging systems : X-ray imaging
(340.7440) X-ray optics : X-ray imaging
(110.4155) Imaging systems : Multiframe image processing
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
X-ray Optics

History
Original Manuscript: November 7, 2013
Manuscript Accepted: January 2, 2014
Published: January 28, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Kartik Ayyer, Hugh T. Philipp, Mark W. Tate, Veit Elser, and Sol M. Gruner, "Real-Space x-ray tomographic reconstruction of randomly oriented objects with sparse data frames," Opt. Express 22, 2403-2413 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-22-3-2403


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References

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