OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Towards acousto-optic tissue imaging with nanosecond laser pulses

S.G. Resink, E. Hondebrink, and W. Steenbergen  »View Author Affiliations

Optics Express, Vol. 22, Issue 3, pp. 3564-3571 (2014)

View Full Text Article

Enhanced HTML    Acrobat PDF (1302 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a way to generate acousto-optical signals in timovssue-like media with nanosecond laser pulses. Our method is based on recording and analyzing speckle patterns formed by interaction of nanosecond laser pulses with tissue, without and with simultaneous application of ultrasound. Stroboscopic application allows visualizing the temporal behavior of speckles while the ultrasound is propagating through the medium. We investigate two ways of quantifying the acousto-optic effect, viz. adding and subtracting speckle patterns obtained at various ultrasound phases. Both methods are compared with the existing speckle contrast method using a 2D scan and are found to perform similarly. Our method gives outlook on overcoming the speckle decorrelation problem in acousto-optics, and therefore brings in-vivo acousto-optic measurements one step closer. Furthermore it enables combining acousto-optics and photoacoustics in one setup with a single laser.

© 2014 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(070.1060) Fourier optics and signal processing : Acousto-optical signal processing
(170.1065) Medical optics and biotechnology : Acousto-optics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 6, 2013
Revised Manuscript: January 29, 2014
Manuscript Accepted: January 30, 2014
Published: February 6, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

S.G. Resink, E. Hondebrink, and W. Steenbergen, "Towards acousto-optic tissue imaging with nanosecond laser pulses," Opt. Express 22, 3564-3571 (2014)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. S. G. Resink, A. C. Boccara, W. Steenbergen, “State-of-the art of acousto-optic sensing and imaging of turbid media,” J. Biomed. Opt. 17(4), 040901 (2012). [CrossRef] [PubMed]
  2. D. S. Elson, R. Li, C. Dunsby, R. Eckersley, M. X. Tang, “Ultrasound-mediated optical tomography: a review of current methods,” Interface Focus 1(4), 632–648 (2011). [CrossRef] [PubMed]
  3. K. Daoudi, A. Hussain, E. Hondebrink, W. Steenbergen, “Correcting photoacoustic signals for fluence variations using acousto-optic modulation,” Opt. Express 20(13), 14117–14129 (2012). [CrossRef] [PubMed]
  4. A. Hussain, K. Daoudi, E. Hondebrink, and W. Steenbergen, “Quantitative Photoacoustic Imaging by Acousto-Optically Measured Light Fluence,” in OSA Technical Digest (Optical Society of America, 2012), BM2B.5.
  5. L. H. V. Wang, “Mechanisms of ultrasonic modulation of multiply scattered coherent light: An analytic model,” Phys Rev Lett. 87(4), 043903 (2001).
  6. M. Lesaffre, F. Jean, F. Ramaz, A. C. Boccara, M. Gross, P. Delaye, G. Roosen, “In situ monitoring of the photorefractive response time in a self-adaptive wavefront holography setup developed for acousto-optic imaging,” Opt. Express 15(3), 1030–1042 (2007). [CrossRef] [PubMed]
  7. S. Lévêque-Fort, “Three-dimensional acousto-optic imaging in biological tissues with parallel signal processing,” Appl. Opt. 40(7), 1029–1036 (2000). [CrossRef] [PubMed]
  8. J. Li, G. Ku, L. V. Wang, “Ultrasound-modulated optical tomography of biological tissue by use of contrast of laser speckles,” Appl. Opt. 41(28), 6030–6035 (2002). [CrossRef] [PubMed]
  9. W. Leutz, G. Maret, “Ultrasonic modulation of multiply scattered-light,” Physica B 204(1-4), 14–19 (1995). [CrossRef]
  10. L. H. Wang, S. L. Jacques, X. M. Zhao, “Continuous-wave ultrasonic modulation of scattered laser light to image objects in turbid media,” Opt. Lett. 20(6), 629–631 (1995). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

Supplementary Material

» Media 1: MOV (3067 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited