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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Axial super-localisation using rotating point spread functions shaped by polarisation-dependent phase modulation

Clemens Roider, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 22, Issue 4, pp. 4029-4037 (2014)

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We present an approach for point spread function (PSF) engineering that allows one to shape the optical wavefront independently in both polarisation directions, with two adjacent phase masks displayed on a single liquid-crystal spatial light modulator (LC-SLM). The set-up employs a polarising beam splitter and a geometric image rotator to rectify and process both polarisation directions detected by the camera. We shape a single-lobe (“corkscrew”) PSF that rotates upon defocus for each polarisation channel and combine the two polarisation channels with a relative 180° phase-shift on the computer, merging them into a single PSF that exhibits two lobes whose orientation contains information about the axial position. A major advantage lies in the possibility to measure and eliminate the aberrations in the two polarisation channels independently. We demonstrate axial super-localisation of isotropically emitting fluorescent nanoparticles. Our implementation of the single-lobe PSFs follows the method proposed by Prasad [Opt. Lett. 38, 585 (2013)], and thus is to the best of our knowledge the first experimental realisation of this suggestion. For comparison we also study an approach with a rotating double-helix PSFs (in only one polarisation channel) and ascertain the trade-off between localisation precision and axial working range.

© 2014 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.0110) Imaging systems : Imaging systems
(110.4850) Imaging systems : Optical transfer functions
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(230.6120) Optical devices : Spatial light modulators
(110.7348) Imaging systems : Wavefront encoding

ToC Category:
Imaging Systems

Original Manuscript: October 22, 2013
Revised Manuscript: December 23, 2013
Manuscript Accepted: December 23, 2013
Published: February 13, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Clemens Roider, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte, "Axial super-localisation using rotating point spread functions shaped by polarisation-dependent phase modulation," Opt. Express 22, 4029-4037 (2014)

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