OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Axial super-localisation using rotating point spread functions shaped by polarisation-dependent phase modulation

Clemens Roider, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations


Optics Express, Vol. 22, Issue 4, pp. 4029-4037 (2014)
http://dx.doi.org/10.1364/OE.22.004029


View Full Text Article

Enhanced HTML    Acrobat PDF (2101 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present an approach for point spread function (PSF) engineering that allows one to shape the optical wavefront independently in both polarisation directions, with two adjacent phase masks displayed on a single liquid-crystal spatial light modulator (LC-SLM). The set-up employs a polarising beam splitter and a geometric image rotator to rectify and process both polarisation directions detected by the camera. We shape a single-lobe (“corkscrew”) PSF that rotates upon defocus for each polarisation channel and combine the two polarisation channels with a relative 180° phase-shift on the computer, merging them into a single PSF that exhibits two lobes whose orientation contains information about the axial position. A major advantage lies in the possibility to measure and eliminate the aberrations in the two polarisation channels independently. We demonstrate axial super-localisation of isotropically emitting fluorescent nanoparticles. Our implementation of the single-lobe PSFs follows the method proposed by Prasad [Opt. Lett. 38, 585 (2013)], and thus is to the best of our knowledge the first experimental realisation of this suggestion. For comparison we also study an approach with a rotating double-helix PSFs (in only one polarisation channel) and ascertain the trade-off between localisation precision and axial working range.

© 2014 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.0110) Imaging systems : Imaging systems
(110.4850) Imaging systems : Optical transfer functions
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(230.6120) Optical devices : Spatial light modulators
(110.7348) Imaging systems : Wavefront encoding

ToC Category:
Imaging Systems

History
Original Manuscript: October 22, 2013
Revised Manuscript: December 23, 2013
Manuscript Accepted: December 23, 2013
Published: February 13, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Clemens Roider, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte, "Axial super-localisation using rotating point spread functions shaped by polarisation-dependent phase modulation," Opt. Express 22, 4029-4037 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-22-4-4029


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth. 5, 527–529 (2008). [CrossRef]
  2. B. Huang, W. Wang, M. Bates, X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319(5864):810–813 (2008). [CrossRef] [PubMed]
  3. S. Pavani, R. Piestun, “Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system,” Opt. Express 16, 22048–22057 (2008). [CrossRef] [PubMed]
  4. S. Pavani, J. DeLuca, R. Piestun, “Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system,” Opt. Express 17, 19644–19655 (2009). [CrossRef] [PubMed]
  5. G. Grover, S. Quirin, C. Fiedler, R. Piestun, “Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging,” Biomed. Opt. Express 2, 3010–3020 (2011). [CrossRef] [PubMed]
  6. M. P. Backlund, M. D. Lew, A. S. Backer, S. J. Sahl, G. Grover, A. Agrawal, R. Piestun, W. E. Moerner, “Simultaneous, accurate measurement of the 3D position and orientation of single molecules,” Proc. Natl. Acad. Sci. USA 109, 19087 (2012). [CrossRef] [PubMed]
  7. A. Backer, M. Backlund, M. Lew, W. Moerner, “Single-molecule orientation measurements with a quadrated pupil,” Opt. Lett. 38, 1521–1523 (2013). [CrossRef] [PubMed]
  8. A. Kress, X. Wang, H. Ranchon, J. Savatier, H. Rigneault, P. Ferrand, S. Brasselet, “Mapping the local organization of cell membranes using excitation-polarization-resolved confocal fluorescence microscopy,” Biophys. J. 105(1), 127–136 (2013). [CrossRef] [PubMed]
  9. S. Prasad, “Rotating point spread function via pupil-phase engineering,” Opt. Lett. 38, 585–587 (2013). [CrossRef] [PubMed]
  10. M. Neil, M. Booth, T. Wilson, “New modal wave-front sensor: a theoretical analysis,” J. Opt. Soc. Am. A 17, 1098–1107 (2000). [CrossRef]
  11. J. Enderlein, “Theoretical study of detecting a dipole emitter through an objective with high numerical aperture,” Opt. Lett. 25, 634–636 (2000). [CrossRef]
  12. B. Richards, E. Wolf, “The airy pattern in systems of high angular aperture,” Proc. Phys. Soc. B 69, 854 (1956). [CrossRef]
  13. C. J. R. Sheppard, P. Torok, “Electromagnetic field in the focal region of an electric dipole wave,” Optik 104175–177 (1997).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited