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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Dynamic stereo microscopy for studying particle sedimentation

M. P. Lee, G. M. Gibson, D. Phillips, M. J. Padgett, and M. Tassieri  »View Author Affiliations


Optics Express, Vol. 22, Issue 4, pp. 4671-4677 (2014)
http://dx.doi.org/10.1364/OE.22.004671


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Abstract

We demonstrate a new method for measuring the sedimentation of a single colloidal bead by using a combination of optical tweezers and a stereo microscope based on a spatial light modulator. We use optical tweezers to raise a micron-sized silica bead to a fixed height and then release it to observe its 3D motion while it sediments under gravity. This experimental procedure provides two independent measurements of bead diameter and a measure of Faxén’s correction, where the motion changes due to presence of the boundary.

© 2014 Optical Society of America

OCIS Codes
(150.6910) Machine vision : Three-dimensional sensing
(230.6120) Optical devices : Spatial light modulators
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Microscopy

History
Original Manuscript: December 19, 2013
Revised Manuscript: February 6, 2014
Manuscript Accepted: February 8, 2014
Published: February 20, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Citation
M. P. Lee, G. M. Gibson, D. Phillips, M. J. Padgett, and M. Tassieri, "Dynamic stereo microscopy for studying particle sedimentation," Opt. Express 22, 4671-4677 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-22-4-4671


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