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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

Effects of spatial coherence in diffraction phase microscopy

Chris Edwards, Basanta Bhaduri, Tan Nguyen, Benjamin G. Griffin, Hoa Pham, Taewoo Kim, Gabriel Popescu, and Lynford L. Goddard  »View Author Affiliations


Optics Express, Vol. 22, Issue 5, pp. 5133-5146 (2014)
http://dx.doi.org/10.1364/OE.22.005133


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Abstract

Quantitative phase imaging systems using white light illumination can exhibit lower noise figures than laser-based systems. However, they can also suffer from object-dependent artifacts, such as halos, which prevent accurate reconstruction of the surface topography. In this work, we show that white light diffraction phase microscopy using a standard halogen lamp can produce accurate height maps of even the most challenging structures provided that there is proper spatial filtering at: 1) the condenser to ensure adequate spatial coherence and 2) the output Fourier plane to produce a uniform reference beam. We explain that these object-dependent artifacts are a high-pass filtering phenomenon, establish design guidelines to reduce the artifacts, and then apply these guidelines to eliminate the halo effect. Since a spatially incoherent source requires significant spatial filtering, the irradiance is lower and proportionally longer exposure times are needed. To circumvent this tradeoff, we demonstrate that a supercontinuum laser, due to its high radiance, can provide accurate measurements with reduced exposure times, allowing for fast dynamic measurements.

© 2014 Optical Society of America

OCIS Codes
(030.1640) Coherence and statistical optics : Coherence
(070.6110) Fourier optics and signal processing : Spatial filtering
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.4630) Instrumentation, measurement, and metrology : Optical inspection
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.0110) Medical optics and biotechnology : Imaging systems

ToC Category:
Coherence and Statistical Optics

History
Original Manuscript: December 23, 2013
Revised Manuscript: February 12, 2014
Manuscript Accepted: February 19, 2014
Published: February 26, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Chris Edwards, Basanta Bhaduri, Tan Nguyen, Benjamin G. Griffin, Hoa Pham, Taewoo Kim, Gabriel Popescu, and Lynford L. Goddard, "Effects of spatial coherence in diffraction phase microscopy," Opt. Express 22, 5133-5146 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-22-5-5133


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