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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

7-ps optical pulse generation from a 1064-nm gain-switched laser diode and its application for two-photon microscopy

Yuta Kusama, Yuichiro Tanushi, Masami Yokoyama, Ryosuke Kawakami, Terumasa Hibi, Yuichi Kozawa, Tomomi Nemoto, Shunichi Sato, and Hiroyuki Yokoyama  »View Author Affiliations


Optics Express, Vol. 22, Issue 5, pp. 5746-5753 (2014)
http://dx.doi.org/10.1364/OE.22.005746


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Abstract

In this study, we investigated the picosecond optical pulse generation from a 1064-nm distributed feedback laser diode under strong gain switching. The spectrum of the generated optical pulses was manipulated in two different ways: (i) by extracting the short-wavelength components of the optical pulse spectrum and (ii) by compensating for spectral chirping in the extracted mid-spectral region. Both of these methods shortened the optical pulse duration to approximately 7 ps. These optical pulses were amplified to over 20-kW peak power for two-photon microscopy. We obtained clear two-photon images of neurons in a fixed brain slice of H-line mouse expressing enhanced yellow fluorescent protein. Furthermore, a successful experiment was also confirmed for in vivo deep region H-line mouse brain neuron imaging.

© 2014 Optical Society of America

OCIS Codes
(140.3070) Lasers and laser optics : Infrared and far-infrared lasers
(140.3490) Lasers and laser optics : Lasers, distributed-feedback
(140.5960) Lasers and laser optics : Semiconductor lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(140.3538) Lasers and laser optics : Lasers, pulsed

ToC Category:
Lasers and Laser Optics

History
Original Manuscript: November 29, 2013
Revised Manuscript: January 16, 2014
Manuscript Accepted: January 16, 2014
Published: March 5, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Yuta Kusama, Yuichiro Tanushi, Masami Yokoyama, Ryosuke Kawakami, Terumasa Hibi, Yuichi Kozawa, Tomomi Nemoto, Shunichi Sato, and Hiroyuki Yokoyama, "7-ps optical pulse generation from a 1064-nm gain-switched laser diode and its application for two-photon microscopy," Opt. Express 22, 5746-5753 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-22-5-5746


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