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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

Multi-color fluorescence imaging based on plasmonic wavelength selection and double illumination by white light

Jong Min Choi, Kyungsik Eom, Seoyoung Hwang, Yena Lee, Sang Beom Jun, Kyung Min Byun, and Sung June Kim  »View Author Affiliations

Optics Express, Vol. 22, Issue 5, pp. 5977-5985 (2014)

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We demonstrate the proof-of-concept for developing a multi-color fluorescence imaging system based on plasmonic wavelength selection and double illumination by white light source. This technique is associated with fluorescence excitation by transmitted light via a diffraction of propagating surface plasmons. Since double illumination through both sides of isosceles triangle prism in the Kretschmann configuration enables multiple transmission beams of different wavelengths to interact with the specimen, our approach can be an alternative to conventional fluorescence detection owing to alignment stability and functional expandability. After fabricating a plasmonic wavelength splitter and integrating it with microscopic imaging system, we successfully confirm the performance by visualizing in vitro neuron cells labeled with green and red fluorescence dyes. The suggested method has a potential that it could be combined with plasmonic biosensor scheme to realize a multi-functional platform which allows imaging and sensing of biological samples at the same time.

© 2014 Optical Society of America

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(170.0110) Medical optics and biotechnology : Imaging systems
(180.2520) Microscopy : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Imaging Systems

Original Manuscript: January 2, 2014
Revised Manuscript: February 23, 2014
Manuscript Accepted: February 24, 2014
Published: March 6, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Jong Min Choi, Kyungsik Eom, Seoyoung Hwang, Yena Lee, Sang Beom Jun, Kyung Min Byun, and Sung June Kim, "Multi-color fluorescence imaging based on plasmonic wavelength selection and double illumination by white light," Opt. Express 22, 5977-5985 (2014)

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