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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction

P. Ferraro, D. Alferi, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini  »View Author Affiliations


Optics Letters, Vol. 31, Issue 10, pp. 1405-1407 (2006)
http://dx.doi.org/10.1364/OL.31.001405


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Abstract

Combining the concept of lateral shear interferometry (LSI) within a digital holography microscope, we demonstrate that it is possible to obtain quantitative optical phase measurement in microscopy by a new single-image-processing procedure. Numerical lateral shear of the reconstructed wavefront in the image plane makes it possible to retrieve the derivative of the wavefront and remove the defocus aberration term introduced by the microscope objective. The method is tested to investigate a silicon structure and a mouse cell line.

© 2006 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(090.1000) Holography : Aberration compensation
(100.2000) Image processing : Digital image processing
(110.1650) Imaging systems : Coherence imaging
(170.1650) Medical optics and biotechnology : Coherence imaging

ToC Category:
Holography

History
Original Manuscript: October 13, 2005
Revised Manuscript: November 17, 2005
Manuscript Accepted: November 18, 2005

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
P. Ferraro, D. Alferi, S. De Nicola, L. De Petrocellis, A. Finizio, and G. Pierattini, "Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction," Opt. Lett. 31, 1405-1407 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-10-1405

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