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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Ex vivo characterization of sub-10-fs pulses

Adam M. Larson and Alvin T. Yeh  »View Author Affiliations

Optics Letters, Vol. 31, Issue 11, pp. 1681-1683 (2006)

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An all-mirror dispersion-compensation setup is used to correct for quadratic and cubic phase distortions induced within a custom nonlinear optical microscope. Mouse tail tendon is used to characterize sub-10-fs pulses by interferometric autocorrelation. This is an ideal method for characterizing dispersion from the optical system, immersion medium, and wet biological sample. The generation of very short autocorrelations demonstrates the ability to compensate for phase distortions within the imaging system and efficient second-harmonic upconversion of the ultrashort pulse spectrum within collagen. Compensated autocorrelation traces are presented for biologically relevant objective lenses.

© 2006 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.6930) Medical optics and biotechnology : Tissue
(170.7160) Medical optics and biotechnology : Ultrafast technology
(320.0320) Ultrafast optics : Ultrafast optics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 13, 2006
Revised Manuscript: March 7, 2006
Manuscript Accepted: March 20, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Adam M. Larson and Alvin T. Yeh, "Ex vivo characterization of sub-10-fs pulses," Opt. Lett. 31, 1681-1683 (2006)

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  1. W. R. Zipfel, R. M. Williams, and W. W. Webb, Nat. Biotechnol. 21, 1369 (2003). [CrossRef] [PubMed]
  2. P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003). [CrossRef] [PubMed]
  3. Z. Bor, Opt. Lett. 14, 119 (1989). [CrossRef] [PubMed]
  4. M. Kempe and W. Rudolph, Opt. Lett. 18, 137 (1993). [CrossRef] [PubMed]
  5. J. B. Guild, C. Xu, and W. W. Webb, Appl. Opt. 36, 397 (1997). [CrossRef] [PubMed]
  6. M. Muller, J. Squier, R. Wolleschensky, U. Simon, and G. J. Brakenhoff, J. Microsc. 191, 141 (1998). [CrossRef] [PubMed]
  7. J. Jasapara and W. Rudolph, Opt. Lett. 24, 777 (1999). [CrossRef]
  8. R. L. Fork, C. H. B. Cruz, P. C. Becker, and C. V. Shank, Opt. Lett. 12, 483 (1987). [CrossRef] [PubMed]
  9. G. J. Brakenhoff, M. Muller, and J. Squier, J. Microsc. 179, 253 (1995). [CrossRef]
  10. V. Iyer, B. E. Losavio, and P. Saggau, J. Biomed. Opt. 8, 460 (2003). [CrossRef] [PubMed]
  11. P. Dombi, V. S. Yakovlev, K. O'Keeffe, T. Fuji, M. Lezius, and G. Tempea, Opt. Express 13, 10888 (2005). [CrossRef] [PubMed]
  12. T. A. Planchon, S. Ferre, G. Hamoniaux, G. Cheriaux, and J.-P. Chambaret, Opt. Lett. 29, 2300 (2004). [CrossRef] [PubMed]
  13. A. G. Van Engen, S. A. Diddams, and T. S. Clement, Appl. Opt. 37, 5679 (1998). [CrossRef]
  14. M. Kempe and W. Rudolph, Phys. Rev. A 48, 4721 (1993). [CrossRef] [PubMed]
  15. M. Kempe, U. Stamm, B. Wilhelmi, and W. Rudolph, J. Opt. Soc. Am. B 9, 1158 (1992). [CrossRef]
  16. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, Nature 432, 822 (2004). [CrossRef] [PubMed]
  17. I. Maxwell, S. Chung, and E. Mazur, Med. Laser. Appl. 20, 193 (2005). [CrossRef]

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