Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples
Optics Letters, Vol. 31, Issue 12, pp. 1830-1832 (2006)
http://dx.doi.org/10.1364/OL.31.001830
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Abstract
We report a Koehler-illumination-based full-field, actively stabilized, low-coherence phase-shifting interferometer, which is built on a white-light Michelson interferometer. By using a phase-stepping technique we can obtain full-field phase images of the sample. An actively stabilized phase-lock circuit is employed in the system to reduce phase noise. An application to human epithelial cells (HeLa cells) is achieved in our experiment. The advancement of this technique rests in its ability to take images of unstained biological samples quantitatively and on a nanometer scale.
© 2006 Optical Society of America
OCIS Codes
(030.1640) Coherence and statistical optics : Coherence
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.4500) Medical optics and biotechnology : Optical coherence tomography
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: February 13, 2006
Revised Manuscript: March 28, 2006
Manuscript Accepted: March 28, 2006
Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics
Citation
Xinhong Li, Toyohiko Yamauchi, Hidenao Iwai, Yutaka Yamashita, Haijun Zhang, and Teruo Hiruma, "Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples," Opt. Lett. 31, 1830-1832 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-12-1830
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