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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy

Feruz Ganikhanov, Conor L. Evans, Brian G. Saar, and X. Sunney Xie  »View Author Affiliations


Optics Letters, Vol. 31, Issue 12, pp. 1872-1874 (2006)
http://dx.doi.org/10.1364/OL.31.001872


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Abstract

We demonstrate a new approach to coherent anti-Stokes Raman scattering (CARS) microscopy that significantly increases the detection sensitivity. CARS signals are generated by collinearly overlapped, tightly focused, and raster scanned pump and Stokes laser beams, whose difference frequency is rapidly modulated. The resulting amplitude modulation of the CARS signal is detected through a lock-in amplifier. This scheme efficiently suppresses the nonresonant background and allows for the detection of far fewer vibrational oscillators than possible through existing CARS microscopy methods.

© 2006 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Microscopy

History
Original Manuscript: January 9, 2006
Revised Manuscript: March 22, 2006
Manuscript Accepted: March 23, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Feruz Ganikhanov, Conor L. Evans, Brian G. Saar, and X. Sunney Xie, "High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy," Opt. Lett. 31, 1872-1874 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-12-1872


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References

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