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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 10 — Oct. 24, 2006

Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging

Sung-Jan Lin, Shiou-Hwa Jee, Chien-Jui Kuo, Ruei-Jr Wu, Wei-Chou Lin, Jau-Shiuh Chen, Yi-Hua Liao, Chih-Jung Hsu, Tsen-Fang Tsai, Yang-Fang Chen, and Chen-Yuan Dong  »View Author Affiliations


Optics Letters, Vol. 31, Issue 18, pp. 2756-2758 (2006)
http://dx.doi.org/10.1364/OL.31.002756


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Abstract

We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and are replaced by autofluorescent signals, indicating that normal collagen structures responsible for SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mohs’ surgery in the real-time guidance of the secure removal of basal cell carcinoma.

© 2006 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 20, 2006
Revised Manuscript: June 24, 2006
Manuscript Accepted: July 3, 2006
Published: August 25, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Sung-Jan Lin, Shiou-Hwa Jee, Chien-Jui Kuo, Ruei-Jr Wu, Wei-Chou Lin, Jau-Shiuh Chen, Yi-Hua Liao, Chih-Jung Hsu, Tsen-Fang Tsai, Yang-Fang Chen, and Chen-Yuan Dong, "Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging," Opt. Lett. 31, 2756-2758 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-18-2756

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