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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 11 — Nov. 13, 2006

In vivo assessment of human skin aging by multiphoton laser scanning tomography

Martin Johannes Koehler, Karsten König, Peter Elsner, Rainer Bückle, and Martin Kaatz  »View Author Affiliations

Optics Letters, Vol. 31, Issue 19, pp. 2879-2881 (2006)

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Changes of dermal collagen and elastin content are characteristic for skin aging as well as for pathological skin conditions. To evaluate these changes, we used in vivo multiphoton laser tomography to measure two-photon excited autofluorescence (AF) and second harmonic generation (SHG). We tested 18 patients of all ages and calculated the SHG-to-AF aging index of dermis (SAAID). We observed a negative relationship between the SAAID and age, which was accelerated for the female ( n = 7 ) subgroup. The current findings are the first in vivo demonstration of this relationship, and they show that specific characteristics of aged skin such as the ratio of extracellular matrix components collagen and elastin can be evaluated by in vivo AF and SHG measurements using near-IR femtosecond laser pulses.

© 2006 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(270.4180) Quantum optics : Multiphoton processes

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 5, 2006
Revised Manuscript: July 14, 2006
Manuscript Accepted: July 18, 2006
Published: September 11, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Martin Johannes Koehler, Karsten König, Peter Elsner, Rainer Bückle, and Martin Kaatz, "In vivo assessment of human skin aging by multiphoton laser scanning tomography," Opt. Lett. 31, 2879-2881 (2006)

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