We demonstrate the use of a simple tool to simultaneously visualize and characterize chromatic and spherical aberrations that are present in multiphoton microscopy. Using two-dimensional Fourier transform spectral interferometry, we measured these aberrations, deducing in a single shot spatiotemporal effects in high-numerical-aperture objectives.
© 2006 Optical Society of America
Original Manuscript: March 27, 2006
Revised Manuscript: July 5, 2006
Manuscript Accepted: July 11, 2006
Published: September 11, 2006
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics
W. Amir, T. A. Planchon, C. G. Durfee, J. A. Squier, P. Gabolde, R. Trebino, and M. Müller, "Simultaneous visualization of spatial and chromatic aberrations by two-dimensional Fourier transform spectral interferometry," Opt. Lett. 31, 2927-2929 (2006)