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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 11 — Nov. 13, 2006

Simultaneous fluorometry and phosphorometry of Langendorff perfused rat heart: ex vivo animal studies

Mahsa Ranji, Dwight L. Jaggard, Sofia V. Apreleva, Sergei A. Vinogradov, and Britton Chance  »View Author Affiliations


Optics Letters, Vol. 31, Issue 20, pp. 2995-2997 (2006)
http://dx.doi.org/10.1364/OL.31.002995


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Abstract

Fluorescence imaging of intrinsic fluorophores of tissue is a powerful method to assess metabolic changes at the cellular and intracellular levels. At the same time, exogenous phosphorescent probes can be used to accurately measure intravascular tissue oxygenation. Heart failure is the leading cause of death in America. A rat heart can potentially model the human heart to study failures or other abnormalities optically. We report simultaneous fluorescence and phosphorescence measurements performed on a rat heart. We have used two different optical systems to acquire fluorescence signals of flavoprotein and nicotinamide adenine dinucleotide—the two intrinsic fluorophores of mitochondria—and the phosphorescence signal of an intravascular oxygen probe to extract intracellular and intravascular metabolism loads, respectively.

© 2006 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 17, 2006
Revised Manuscript: July 11, 2006
Manuscript Accepted: July 25, 2006
Published: September 25, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Mahsa Ranji, Dwight L. Jaggard, Sofia V. Apreleva, Sergei A. Vinogradov, and Britton Chance, "Simultaneous fluorometry and phosphorometry of Langendorff perfused rat heart: ex vivo animal studies," Opt. Lett. 31, 2995-2997 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-20-2995


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References

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