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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 1 — Jan. 19, 2007

In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents

Vladimir P. Zharov, Ekaterina I. Galanzha, Evgeny V. Shashkov, Nicolai G. Khlebtsov, and Valery V. Tuchin  »View Author Affiliations


Optics Letters, Vol. 31, Issue 24, pp. 3623-3625 (2006)
http://dx.doi.org/10.1364/OL.31.003623


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Abstract

A new photoacoustic flow cytometry was developed for real-time detection of circulating cells, nanoparticles, and contrast agents in vivo. Its capability, integrated with photothermal and optical clearing methods, was demonstrated using a near-infrared tunable laser to characterize the in vivo kinetics of Indocyanine Green alone and single cancer cells labeled with gold nanorods and Indocyanine Green in the vasculature of the mouse ear. In vivo applications are discussed, including selective nanophotothermolysis of metastatic squamous cells, label-free detection of melanoma cells, study of pharmokinetics, and immune response to apoptotic and necrotic cells, with potential translation to humans. The threshold sensitivity is estimated as one cancer cell in the background of 10 7 normal blood cells.

© 2006 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 17, 2006
Revised Manuscript: September 7, 2006
Manuscript Accepted: October 2, 2006
Published: November 22, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Vladimir P. Zharov, Ekaterina I. Galanzha, Evgeny V. Shashkov, Nicolai G. Khlebtsov, and Valery V. Tuchin, "In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents," Opt. Lett. 31, 3623-3625 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-24-3623


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References

  1. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, Opt. Lett. 29, 77 (2004). [CrossRef] [PubMed]
  2. V. Zharov, E. Galanzha, and V. Tuchin, in Proc. SPIE 5320, 256 (2004).
  3. V. P. Zharov, E. I. Galanzha, and V. V. Tuchin, J. Cell. Biochem. 97, 916 (2006). [CrossRef] [PubMed]
  4. X. Wang, G. Ku, M. A. Wegiel, D. J. Bornhop, G. Stoica, and L. V. Wang, Opt. Lett. 29, 730 (2004). [CrossRef] [PubMed]
  5. X. Wei, J. M. Runnels, and C. P. Lin, Invest. Ophthalmol. Visual Sci. 44, 4489 (2003). [CrossRef]

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