Dual-pump coherent anti-Stokes-Raman scattering microscopy
Optics Letters, Vol. 31, Issue 24, pp. 3656-3658 (2006)
http://dx.doi.org/10.1364/OL.31.003656
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Abstract
We introduce dual-pump coherent anti-Stokes–Raman scattering (dual-CARS) microscopy. This new technique permits simultaneous imaging of two species characterized by different molecular vibrations, as well as the removal of nonresonant background. This is achieved by using three synchronized laser pulses probing two different vibrations. We demonstrate the virtues of the method by imaging a mixture of nondeuterated and deuterated lipids, clearly distinguishing the individual components and their organization in the mixed arrangement. Further, dual-CARS images of lipid stores in living Caenorhabditis elegans nematodes show that the suppression of the nonresonant background results in significantly enhanced image contrast.
© 2006 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
ToC Category:
Spectroscopy
History
Original Manuscript: August 22, 2006
Revised Manuscript: September 28, 2006
Manuscript Accepted: September 28, 2006
Published: November 22, 2006
Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics
Citation
Ondrej Burkacky, Andreas Zumbusch, Christian Brackmann, and Annika Enejder, "Dual-pump coherent anti-Stokes-Raman scattering microscopy," Opt. Lett. 31, 3656-3658 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-24-3656
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