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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 4 — Apr. 12, 2006

Efficient in-depth trapping with an oil-immersion objective lens

S. NaderS. Reihani, Mohammad A. Charsooghi, Hamid R. Khalesifard, and Ramin Golestanian  »View Author Affiliations


Optics Letters, Vol. 31, Issue 6, pp. 766-768 (2006)
http://dx.doi.org/10.1364/OL.31.000766


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Abstract

Maximum trapping efficiency in optical tweezers occurs close to the coverslip because spherical aberration owing to a mismatch in the refractive indices of the specimen (water) and the immersion oil dramatically decreases the trap efficiency as the trap depth increases. Measuring the axial trap efficiency at various tube lengths by use of an oil-immersion objective has shown that such an aberration can be balanced by another source of spherical aberration, leading to a shift in the position of the maximum efficiency in the Z direction. For a 1.1 μ m polystyrene bead we could achieve the maximal efficiency at a depth of 70 μ m , whereas the trap was stable up to a depth of 100 μ m .

© 2006 Optical Society of America

OCIS Codes
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(140.7010) Lasers and laser optics : Laser trapping

ToC Category:
Trapping

History
Original Manuscript: September 12, 2005
Revised Manuscript: November 29, 2005
Manuscript Accepted: December 8, 2005

Virtual Issues
Vol. 1, Iss. 4 Virtual Journal for Biomedical Optics

Citation
S. Nader S. Reihani, Mohammad A. Charsooghi, Hamid R. Khalesifard, and Ramin Golestanian, "Efficient in-depth trapping with an oil-immersion objective lens," Opt. Lett. 31, 766-768 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-6-766


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References

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  9. The working distance for an objective lens is defined as the distance between the specimen and the front surface of the objective lens. Whereas a typical value of this parameter for a normal objective is ∼100 μm, for long-working-distance objective lenses it could be more than 200 μm.

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