Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy
Optics Letters, Vol. 31, Issue 7, pp. 945-947 (2006)
http://dx.doi.org/10.1364/OL.31.000945
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Abstract
Standing-wave total-internal-reflection fluorescence (SW-TIRF) microscopy uses a super-diffraction-limited standing evanescent wave to extract the high-spatial-frequency content of an object through a diffraction-limited optical imaging system. The effective point-spread function is better than a quarter of the emission wavelength. With a 1.45 numerical aperture objective and 532 nm excitation wavelength, a Rayleigh resolution of approximately 100 nm can be achieved, which is better than twice the resolution of conventional TIRF microscopy. This first experimental realization of SW-TIRF in an objective-launched geometry demonstrates the potential for extended resolution imaging at high speed by using wide-field microscopy.
© 2006 Optical Society of America
OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy
ToC Category:
Microscopy
History
Original Manuscript: November 4, 2005
Revised Manuscript: December 17, 2005
Manuscript Accepted: December 19, 2005
Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Euiheon Chung, Daekeun Kim, and Peter T. So, "Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy," Opt. Lett. 31, 945-947 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-7-945
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