Standing-wave total-internal-reflection fluorescence (SW-TIRF) microscopy uses a super-diffraction-limited standing evanescent wave to extract the high-spatial-frequency content of an object through a diffraction-limited optical imaging system. The effective point-spread function is better than a quarter of the emission wavelength. With a 1.45 numerical aperture objective and 532 nm excitation wavelength, a Rayleigh resolution of approximately 100 nm can be achieved, which is better than twice the resolution of conventional TIRF microscopy. This first experimental realization of SW-TIRF in an objective-launched geometry demonstrates the potential for extended resolution imaging at high speed by using wide-field microscopy.
© 2006 Optical Society of America
Original Manuscript: November 4, 2005
Revised Manuscript: December 17, 2005
Manuscript Accepted: December 19, 2005
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics
Euiheon Chung, Daekeun Kim, and Peter T. So, "Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy," Opt. Lett. 31, 945-947 (2006)