The characterization and control of the phase of broadband femtosecond pulses in nonlinear microscopy are successfully demonstrated with a collinear configuration of spectral shear interferometry for direct electric field reconstruction (SPIDER). A femtosecond-pulse shaper is used as a dispersionless interferometer for the measurement of the spectral phase and to actively compress a broadband supercontinuum from a photonic crystal fiber. This allows in situ online phase management and enables the application of quantum control spectroscopy in microenvironments.
© 2006 Optical Society of America
Original Manuscript: December 5, 2005
Manuscript Accepted: January 9, 2006
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics
Bernhard von Vacano, Tiago Buckup, and Marcus Motzkus, "In situ broadband pulse compression for multiphoton microscopy using a shaper-assisted collinear SPIDER," Opt. Lett. 31, 1154-1156 (2006)