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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation

F. Chasles, B. Dubertret, and A. C. Boccara  »View Author Affiliations


Optics Letters, Vol. 31, Issue 9, pp. 1274-1276 (2006)
http://dx.doi.org/10.1364/OL.31.001274


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Abstract

We present a technique for imaging fluorescent particles based on the axial modulation of the objective’s focal plane position. This technique provides full-field optical sectioning and can be used to localize the fluorophores in three dimensions. We describe the technique and apply it to image 200 nm diameter fluorescent beads immobilized in a gel. We show that full-field optical sectioning is obtained and that the beads are localized with a precision of 10 nm in the transverse plane and 14 nm in the axial direction.

© 2006 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 30, 2005
Revised Manuscript: February 1, 2006
Manuscript Accepted: February 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
F. Chasles, B. Dubertret, and A. C. Boccara, "Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation," Opt. Lett. 31, 1274-1276 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-9-1274

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