Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation
Optics Letters, Vol. 31, Issue 9, pp. 1274-1276 (2006)
http://dx.doi.org/10.1364/OL.31.001274
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Abstract
We present a technique for imaging fluorescent particles based on the axial modulation of the objective’s focal plane position. This technique provides full-field optical sectioning and can be used to localize the fluorophores in three dimensions. We describe the technique and apply it to image
© 2006 Optical Society of America
OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
ToC Category:
Microscopy
History
Original Manuscript: November 30, 2005
Revised Manuscript: February 1, 2006
Manuscript Accepted: February 1, 2006
Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics
Citation
F. Chasles, B. Dubertret, and A. C. Boccara, "Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation," Opt. Lett. 31, 1274-1276 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-9-1274
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