The use of an optically thin indium–tin–oxide (ITO) electrode is presented for an optoelectric biosensor simultaneously recording optical images and microimpedance to examine time-dependent cellular growth. The transmittance of a $100\mathrm{nm}$ thick ITO electrode layer is approximately the same as the transmittance of a clean glass substrate, whereas the industry-standard $\mathrm{Au}\left(47.5\mathrm{nm}\right)∕\mathrm{Ti}\left(2.5\mathrm{nm}\right)$ electrode layer drops the transmittance to less than 10% of that of the glass substrate. The simultaneous optoelectric measurements permit determining the correlation of the cell-covered area increase with the microimpedance increase, and the example results obtained for live porcine pulmonary artery endothelial cells delineate the quantitative and comprehensive nature of cellular attachment and spreading to the substrate, which has not been clearly perceived before.

© 2007 Optical Society of America

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