Simultaneous manipulation and detection of living cell membrane dynamics
Optics Letters, Vol. 32, Issue 13, pp. 1893-1895 (2007)
http://dx.doi.org/10.1364/OL.32.001893
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Abstract
We report a novel optical-tweezers-based method to study the membrane motion at the leading edge of biological cells with nanometer spatial and microsecond temporal resolution. A diffraction-limited laser spot was positioned at the leading edge of a cell, and the forward scattered light was imaged on a quadrant photodiode that served as a position sensitive device. The universality of this technique is demonstrated with different cell types. We investigated the membrane motion at the leading edge of red blood cells in detail and showed that this technique can achieve simultaneous manipulation and detection of cellular edge dynamics with unprecedented precision.
© 2007 Optical Society of America
OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: March 23, 2007
Manuscript Accepted: April 30, 2007
Published: June 22, 2007
Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Michael Gögler, Timo Betz, and Josef Alfons Käs, "Simultaneous manipulation and detection of living cell membrane dynamics," Opt. Lett. 32, 1893-1895 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-13-1893
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