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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Lateral modulation boosts image quality in single plane illumination fluorescence microscopy

Tobias Breuninger, Klaus Greger, and Ernst H.K. Stelzer  »View Author Affiliations


Optics Letters, Vol. 32, Issue 13, pp. 1938-1940 (2007)
http://dx.doi.org/10.1364/OL.32.001938


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Abstract

A new microscope combines optical sectioning by fluorophore excitation using a single light sheet with structured illumination. Several images with laterally intensity-modulated light sheets are recorded from scattering fluorescent specimens. By applying a simple data processing scheme, the nonmodulated volumes are identified. The blurred features become dark, and the resultant images are improved in terms of contrast and resolution. Hence, the instrument is capable of discriminating against contributions to the image that are induced by the optical properties of the specimen. The new microscope’s capabilities are demonstrated by imaging the internals of the head of an adult Drosophila melanogaster (fruit fly) expressing green fluorescent protein–labeled polycomb proteins.

© 2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(260.0260) Physical optics : Physical optics

ToC Category:
Microscopy

History
Original Manuscript: March 29, 2007
Manuscript Accepted: March 30, 2007
Published: June 26, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Tobias Breuninger, Klaus Greger, and Ernst H. K. Stelzer, "Lateral modulation boosts image quality in single plane illumination fluorescence microscopy," Opt. Lett. 32, 1938-1940 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-13-1938


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References

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