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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Aberration-free optical refocusing in high numerical aperture microscopy

Edward J. Botcherby, Rimas Juškaitis, Martin J. Booth, and Tony Wilson  »View Author Affiliations


Optics Letters, Vol. 32, Issue 14, pp. 2007-2009 (2007)
http://dx.doi.org/10.1364/OL.32.002007


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Abstract

We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 μ m for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen.

© 2007 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 5, 2007
Manuscript Accepted: May 18, 2007
Published: July 3, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Edward J. Botcherby, Rimas Juskaitis, Martin J. Booth, and Tony Wilson, "Aberration-free optical refocusing in high numerical aperture microscopy," Opt. Lett. 32, 2007-2009 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-14-2007

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