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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence

Vadim Y. Soloviev, James McGinty, Khadija B. Tahir, Mark A.A. Neil, Alessandro Sardini, Joseph V. Hajnal, Simon R. Arridge, and P. M.W. French  »View Author Affiliations


Optics Letters, Vol. 32, Issue 14, pp. 2034-2036 (2007)
http://dx.doi.org/10.1364/OL.32.002034


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Abstract

We present a novel fluorescence lifetime tomography system applied to a highly scattering autofluorescent phantom containing live cells expressing the fluorophore enhanced green fluorescent protein (EGFP). The fluorescence signal was excited using a fiber-laser-pumped supercontinuum source and detected using wide-field time gating imaging. To facilitate rapid 3D reconstruction of the fluorescence lifetime distribution, the time-resolved data were Fourier-transformed in time to give complex functions that formed a data set for the Fourier domain reconstruction. Initially the presence of an unspecified background autofluorescence signal impeded reconstruction of the lifetime distribution, but we show that this problem can be addressed using a simple iterative technique.

© 2007 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(290.0290) Scattering : Scattering
(290.7050) Scattering : Turbid media

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 1, 2007
Revised Manuscript: May 9, 2007
Manuscript Accepted: May 18, 2007
Published: July 6, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Vadim Y. Soloviev, James McGinty, Khadija B. Tahir, Mark A. Neil, Alessandro Sardini, Joseph V. Hajnal, Simon R. Arridge, and P. M. French, "Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence," Opt. Lett. 32, 2034-2036 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-14-2034

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