Microscope enabling multimodality imaging, angle-resolved scattering, and scattering spectroscopy
Optics Letters, Vol. 32, Issue 16, pp. 2348-2350 (2007)
http://dx.doi.org/10.1364/OL.32.002348
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Abstract
We present the design, construction, and initial characterization of a multifunctional imaging/scattering spectroscopy system built around a commercial inverted microscope platform. The system enables co-registered brightfield, Fourier-filtered darkfield, and fluorescence imaging; monochromatic angle-resolved scattering measurements; and white-light wavelength-resolved scattering spectroscopy from the same field of view. A fiber-based illumination system provides illumination-wavelength flexibility and a good approximation to a point source. The performance of the system in its various data acquisition modes is experimentally verified using fluorescent microspheres. This multifunctional instrument provides a platform for studies on adherent cells from which the biophysical implications of subcellular light scattering can be studied in conjunction with sensitive fluorescence-based techniques.
© 2007 Optical Society of America
OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(290.0290) Scattering : Scattering
(290.5820) Scattering : Scattering measurements
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: May 9, 2007
Manuscript Accepted: June 21, 2007
Published: August 2, 2007
Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics
Citation
W. J. Cottrell, J. D. Wilson, and T. H. Foster, "Microscope enabling multimodality imaging, angle-resolved scattering, and scattering spectroscopy," Opt. Lett. 32, 2348-2350 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-16-2348
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