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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy

Florian Charrière, Anca Marian, Tristan Colomb, Pierre Marquet, and Christian Depeursinge  »View Author Affiliations


Optics Letters, Vol. 32, Issue 16, pp. 2456-2458 (2007)
http://dx.doi.org/10.1364/OL.32.002456


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Abstract

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100 × 1.3 NA MO are presented.

© 2007 Optical Society of America

OCIS Codes
(090.1000) Holography : Aberration compensation
(090.1760) Holography : Computer holography
(110.1220) Imaging systems : Apertures
(110.4850) Imaging systems : Optical transfer functions

ToC Category:
Holography

History
Original Manuscript: June 13, 2007
Manuscript Accepted: July 11, 2007
Published: August 10, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Florian Charrière, Anca Marian, Tristan Colomb, Pierre Marquet, and Christian Depeursinge, "Amplitude point-spread function measurement of high-NA microscope objectives by digital holographic microscopy," Opt. Lett. 32, 2456-2458 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-16-2456

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